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I.M.A.G.E. Consortium cDNA clones

I.M.A.G.E. Consortium cDNA clones

The Integrated Molecular Analysis of Genomes and their Expression (I.M.A.G.E.) Consortium was created in 1993 by four academic groups. The consortium's primary goal is to create arrayed cDNA libraries and associated bioinformatics tools, and make them publicly available to the research community. 

The cDNA libraries were further developed by the MGC to construct library sets of clones containing full length coding sequences, and then by the ORFeome Collaboration to offer ORF entry clones and expression clones.

The primary organisms of interest include human, mouse, rat, bovine, zebrafish, Fugu, Xenopus laevis and Xenopus tropicalis (frog).

IMAGE cDNA clones are classified as:

  • EST Clones - where only the 5' or 3' ends of the insert have been sequenced and these clones may not contain a full coding sequence (cds)
  • cDNA: partial coding sequence (cds) - where the clone insert does not contain a full coding sequence*
  • cDNA: potentially full coding sequence (cds) - where the clone insert potentially contains a full coding sequence*

*The sequences are bioinformatically analysed and identified as either containing the full coding sequence (cds) or only containing part of the coding sequence.

PLEASE NOTE however that this information may not reflect the most recent annotation of the reference sequence and you should carefully check the clone insert sequence shown on NCBI (using the accession number link on the clone webpage) to make sure that it matches the sequence you expect.

 

The I.M.A.G.E. consortium make arrayed cDNA libraries available to the community. Each clone has been given a unique I.M.A.G.E. ID to facilitate reference. The sequence, mapping and expression data has been placed in the public domain. The primary organisms of interest include human, mouse, rat, bovine, zebrafish, Fugu, Xenopus laevis and Xenopus tropicalis (frog). Utilizing high speed robotics, over nine million individual cDNA clones have been arrayed into 384-well microtiter plates.

Protocols / Clone Handling

Clones have been streaked onto LB agar containing the appropriate selective antibiotic.  Clones should be stored at +4°C on receipt.

As soon as possible, re-streak the clones onto the same medium and incubate overnight at 37°C to obtain single colonies.

A selection of at least 10 single colonies should be picked and grown in LB broth containing the appropriate antibiotic + 8 % glycerol for subsequent freezing at -70°C.

These frozen stocks can then be regrown for plasmid isolation and purification.

Streaking out for single colonies is essential in case of contamination or cross-contamination, or if modifications of the original clone have taken place as the library has been replicated. If there is a problem, the more single colonies you streak out, the more likely it is that you can retrieve the original construct. You can also isolate the original clone from a contaminated sample by following this procedure.

References

Lennon, G.G., Auffray, C., Polymeropoulos, M., Soares, M.B. The I.M.A.G.E. Consortium: An Integrated Molecular Analysis of Genomes and their Expression. Genomics 33:151-152 [1996].


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