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Human Minimal Genomic Set

Human Minimal Genomic Set

The Minimal Genomic Clone Set was developed on basis of NCBI's genome assembly and the Ensembl annotation. The current clone set consists of 25,130 clones. The majority (22,898 clones, 91%) of which have been completely sequenced during the human genome sequencing project. Gaps were covered by 2,232 end-sequenced clones as far as such clones were available. We focused on publicly available BAC and PAC clones and completed the set where necessary with cosmids. The overall average insert size is approx 130 kb.

  • Clone Collection in microtiter plates
  • Customized subsets

Protocols / Clone Handling

Individual clones have been streaked onto LB agar containing the appropriate antibiotic. Please store them at 4 °C (not in a freezer).

As soon as possible, transfer the culture to LB broth containing the antibiotic + 8% glycerol and incubate overnight at 37 °C (do not try to transfer single colonies, instead scoop out the whole bacterial culture using a disposable inoculation loop).

Incubate overnight at 37 °C for subsequent freezing at -70 °C.

References

Candidate Genes in Breast Cancer Revealed by Microarray-Based Comparative Genomic Hybridization of Archived Tissue
Cancer Research 65, 439-447, January 15, 2005

Detection of Chromosomal Imbalances in Retinoblastoma by Matrix-Based Comparative Genomic Hybridization
CANCER 43:294-301 (2005)


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