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Mouse PAC (RPCI-21)

Mouse PAC (RPCI-21)

This mouse PAC library RPCI21 was constructed by Kazutoyo Osoegawa working in Pieter de Jongs' laboratory at the Roswell Park Cancer Institute, Buffalo. The vector, pPAC4, was constructed by Eirik Frengen. The source is female 129/SvevTACfBr mouse spleen genomic DNA. The average insert size is 147 Kbp.

Further information on the library and the vector can be obtained from the WWW site: http://bacpacresources.org//

The library consists of approx. 128,000 clones in 672 microtitre plates (384-well format). The plate numbers run from 337 to 672 . 

The library is available from us as high density gridded filters for screening by hybridisation. The set consists of 7 double spotted 22.2 x 22.2 cm filters.

Protocols / Clone Handling

Clones are streaked onto LB agar containing kanamycin (25µg/ml).

To use: restreak the clone onto the same agar type, in such a way as to obtain single colonies. Incubate at 37°C overnight.

Single colonies are then ready for DNA isolation, if required, using the accompanying protocol.

Clones can be grown in LB broth + kanamycin, and glycerol added after growth to a concentration of 25% for long term storage at -70°C.

 

References

K. Osoegawa et al [2000] "Bacterial artificial chromosome libraries for mouse sequencing and functional analysis." Genome Research 10: 116-128

Subsequent publication:

Please acknowledge the originator of the library, Pieter de Jong , and Source BioScience LifeSciences for providing the clone in any publication arising out of using this resource. An example of how a clone (E.g. 471-m19) should be described in a publication or database entry is: RP21-471M19 from the RPCI mouse PAC library 21.


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