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Human miRNA Collection (NKI)

Small, endogenous (miRNAs) regulate gene expression by mechanisms conserved across many multicelluar organisms. Recent studies have suggested that miRNAs have a role in cellular differentiation, tissue specification, apoptosis and the development of various human cancers. Uncovering the functions of the growing list of miRNAs presents a challenge to developmental biologists.

We offer the complete annotated NKI human miRNA library (miR-Lib) . Each fragment is cloned into a retroviral expression vector and is placed under the control of a CMV promoter. The library was constructed using the Rfam release 6 annotated database. Unlike other libraries, this library has tested and proven efficiency and is extremely flexible where constructs can be transduced or transfected.

For more detailed annotation, we advise that you access the Wellcome Trust Sanger Institute microRNA website at: http://microrna.sanger.ac.uk/

 

As part of our sequence verification service, individual clones are end sequenced before they are sent to you. If the requested clone does not match the expected sequence, Source BioScience will not apply any charge and will contact the purchaser to confirm if they wish to cancel the order.This risk free option ensures that you have the right clone, removing the chance of receiving one of the wrong clones that are inherent in some libraries and can also save you time from having to sequence the clone yourself.

PLEASE NOTE – THAT Source Bioscience excludes any warranty pertaining to the accuracy of the sequence for clones orders where the client chooses to reject the offered sequence verification option, as the clones are supplied as-is, direct from the original library.

This library is composed of 371 individual clones. Each fragment of the human miRNA library was cloned into a retroviral expression vector and was placed under the control of a CMV promoter. It was constructed using the Rfam release 6 annotated database. Its large flanking regions enable endogenous regulation of pre-miRNA expression. It has proven human cell expression supporting the validity of use.

We offer the complete clone set of 1 x 384-well microtitre plate or individual clones, for research purposes only. We can also make subsets of your chosen clones from the collection. Please contact us to discuss your requirements.

Protocols / Clone Handling

Please note that the clone(s) are tested as negative in our phage contamination assay prior to shipping. However, these clones should still be handled with care as no phage assay can be guaranteed to be 100% accurate.

Plates contain LB broth with 8% glycerol and ampicillin. The plates are sent on Dry Ice, and should be stored at -70 °C

Individual clones have been streaked onto LB agar containing ampicillin (100 µg/ml). Please store them at 4 °C (not in a freezer). As soon as possible, transfer the culture to LB broth containing ampicillin + 8% glycerol and incubate overnight at 37 °C (do not try to transfer single colonies, instead scoop out the whole bacterial culture using a disposable inoculation loop). Incubate overnight at 37 °C for subsequent freezing at -70 °C.

These clones are for research purposes only.

References

Acknowledgements and subsequent publication:

Please acknowledge the Netherlands Cancer Institute (NKI) and Source BioScience LifeSciences in any resulting publications.

Please also cite the following paper:

Voorhoeve PM, le Sage C, Schrier M, Gillis AJM, Stoop H, Nagel R, Liu YP, van Duijse Y, Drost J, Griekspoor A, Zlotorynski E, Yabuta N, De Vita G,Nojima H, Looijenga, LHJ and Agami R.

A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors.

Cell. 2006 Mar 24;124(6):1169-81.


For further information and prices please contact us or call +44 (0)115 973 9012

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