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Vector pCYPAC2

This is the information supplied by BACPAC Resources about the vector pCYPAC2:

"We have combined the best features of the P1 and BAC systems. The pCYPAC2 vector has been constructed for the cloning of large DNA fragments following electroporation. This vector has been constructed by removing the stuffer fragment form the pAd10SacBII vector (Pierce, J. C., Sauer, B. and Sternberg, N. (1992) Proc. Natl. Acad. Sci, USA 89: 2056-2060.) and inserting a pUC plasmid into the BamHI cloning site.
The pUC sequence has not been inserted in a permanent way. During the cloning process, pUC sequences are removed through a double-digestion scheme, which employs BamHI and ScaI. The pUC plasmid cannot back into the BamHI cloning site, which would otherwise result in the creation of non-recombinant background clones in the library.

Re-ligation into the BamHI site is prevented at three levels:

  1. pUC has been cleaved into ScaI fragments (ends incompatible with BamHI)
  2. The BamHI/ScaI oligo linkers have been physically separated from the pUC and PAC-vector fragments and
  3. All vector derived fragments have been treated with alkaline phosphates to inhibit ligation of vector-to-vector ends pCYPAC2 clones have Kanamycin antibiotic resistance. Clones should be grown in LB containing 25ug Kanamycin/ml.

We have replaced the SciI site with a second NotI site to allow release of most inserts as a single large fragment. This facilitates size determination of inserts as a single large fragment. This facilitates size determination of inserts and determining the completeness of the NotI digest reaction (via appearance of a constant vector product)."

origin:
Roswell Park Cancer Institute, Human Genetics Department, Pieter J. de Jong - Principal Investigator, Elm and Carltin Streets, Buffalo, New York 14263-0001

Related Links:
Bacpac Resources (http://bacpac.med.buffalo.edu/vectorframe.htm)
pCYPAC2
LOCUS       pCYPAC2     18754 bp dsDNA    Circular
DEFINITION  Cloning vector pCYPAC2, complete sequence.
COMMENT This pCYPAC2 enrty is based on GenBank U09128. 
COMMENT Revisions made by Eirik (Lox, Kan & pBR322seq added in feature 
COMMENT table). Sequence reviced after sequencing of a pPAC4-fragment 
COMMENT (G #14081 deleted: restores a PstI-site, C inserted at #9222: 
COMMENT restores a BglI-site, 3 replacements in LoxP #4771-4794) all 
COMMENT in accordance to the old partial sequence from Nat Sternberg.
FEATURES               Location/Qualifiers
     misc_feature    complement(2746..2763)
                     /label=SP6
     rep_origin      4795..12904
                     /note="P1 plasmid replicon "
                     /label=PLASMID REPLICON
     misc_feature    15224..17034
                     /note="Defined based upon the flanking pBR322 seqs"
                     /label=P1 LYTIC REPLICON
     promoter        18677..18717
                     /note="E.coli promoter, C1 binding site"
                     /label=PROMOTER
     misc_feature    18726..18748
                     /note="T7 primer"
                     /label=T7 
     CDS             2862..4280
                     /note="E.coli SacB gene"
                     /label=SACBII
     CDS             complement(13203..14018)
                     /label=KANAMYCIN
     misc_feature    12905..14140
                     /note="Tn903 sequences"
                     /label=TN903
     misc_feature    17035..18680
                     /note="pBR322 bases#652-2301 (3'-end of TcR & ROP)"
                     /label=PBR322-SEQUENCES
     misc_feature    14944..15223
                     /note="pBR322 seqs"
                     /label=PBR322 SEQS
     misc_feature    4362..4741
                     /note="pBR322 SEQs"
                     /label=PBR322 SEQS
     misc_feature    4771..4794
                     /note="LoxP (This is not the "original" LoxP site)"
                     /label=LOXP
     misc_feature    1..2739
                     /label=PUC-LINK
ORIGIN
        1  gatccgggtt taaacccagt actctagatc ctctagagtc gacctgcagg 
      51  catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 
     101  ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 
     151  aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 
     201  tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 
     251  aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 
     301  cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     351  gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 
     401  taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
     451  gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
     501  gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 
     551  actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
     601  ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     651  ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt 
     701  gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
     751  ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
     801  agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 
     851  agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
     901  acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 
     951  gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
    1001  cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
    1051  aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
    1101  cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 
    1151  aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
    1201  tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 
    1251  agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
    1301  ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 
    1351  gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 
    1401  ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 
    1451  tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
    1501  gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 
    1551  acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 
    1601  cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 
    1651  aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 
    1701  gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 
    1751  catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
    1801  cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 
    1851  atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 
    1901  tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 
    1951  gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 
    2001  tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 
    2051  cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
    2101  tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
    2151  ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
    2201  cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 
    2251  tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg 
    2301  cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 
    2351  ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 
    2401  gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca 
    2451  tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 
    2501  cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc 
    2551  tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 
    2601  cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 
    2651  agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcgagc 
    2701  tcggtacccg gggatctaga gtactgggtt taaacccgga tccttctata 
    2751  gtgtcaccta aatgtcgacg gccaggcggc cgccaggcct acccactagt 
    2801  caattcggga ggatcgaaac ggcagatcgc aaaaacagta catacagaag 
    2851  gagacatgaa catgaacatc aaaaaaattg taaaacaagc cacagttctg 
    2901  acttttacga ctgcacttct ggcaggagga gcgactcaag ccttcgcgaa 
    2951  agaaaataac caaaaagcat acaaagaaac gtacggcgtc tctcatatta 
    3001  cacgccatga tatgctgcag atccctaaac agcagcaaaa cgaaaaatac 
    3051  caagtgcctc aattcgatca atcaacgatt aaaaatattg agtctgcaaa 
    3101  aggacttgat gtgtgggaca gctggccgct gcaaaacgct gacggaacag 
    3151  tagctgaata caacggctat cacgttgtgt ttgctcttgc gggaagcccg 
    3201  aaagacgctg atgacacatc aatctacatg ttttatcaaa aggtcggcga 
    3251  caactcaatc gacagctgga aaaacgcggg ccgtgtcttt aaagacagcg 
    3301  ataagttcga cgccaacgat ccgatcctga aagatcagac gcaagaatgg 
    3351  tccggttctg caacctttac atctgacgga aaaatccgtt tattctacac 
    3401  tgactattcc ggtaaacatt acggcaaaca aagcctgaca acagcgcagg 
    3451  taaatgtgtc aaaatctgat gacacactca aaatcaacgg agtggaagat 
    3501  cacaaaacga tttttgacgg agacggaaaa acatatcaga acgttcagca 
    3551  gtttatcgat gaaggcaatt atacatccgg cgacaaccat acgctgagag 
    3601  accctcacta cgttgaagac aaaggccata aataccttgt attcgaagcc 
    3651  aacacgggaa cagaaaacgg ataccaaggc gaagaatctt tatttaacaa 
    3701  agcgtactac ggcggcggca cgaacttctt ccgtaaagaa agccagaagc 
    3751  ttcagcagag cgctaaaaaa cgcgatgctg agttagcgaa cggcgccctc 
    3801  ggtatcatag agttaaataa tgattacaca ttgaaaaaag taatgaagcc 
    3851  gctgatcact tcaaacacgg taactgatga aatcgagcgc gcgaatgttt 
    3901  tcaaaatgaa cggcaaatgg tacttgttca ctgattcacg cggttcaaaa 
    3951  atgacgatcg atggtattaa ctcaaacgat atttacatgc ttggttatgt 
    4001  atcacactct ttaaccggcc cttacaagcc gctgaacaaa acagggcttg 
    4051  tgctgcaaat gggtcttgat ccaaacgatg tgacattcac ttactctcac 
    4101  ttcgcagtgc cgcaagccaa aggcaacaat gtggttatca caagctacat 
    4151  gacaaacaga ggcttcttcg aggataaaaa ggcaacattt gcgccaagct 
    4201  tcttaatgaa catcaaaggc aataaaacat ccgttgtcaa aaacagcatc 
    4251  ctggagcaag gacagctgac agtcaactaa taacagcaaa aagaaaatgc 
    4301  cgatacttca ttggcatttt cttttatttc tcaacaagat ggtgaattga 
    4351  ctagtgggta gatccacagg acgggtgtgg tcgccatgat cgcgtagtcg 
    4401  atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg 
    4451  gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat 
    4501  atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg 
    4551  atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac 
    4601  agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt 
    4651  tcatacacgg tgcctgactg cgtttagcaa ttttaactgt gataaactac 
    4701  cgcattaaag cttatcgatg ataagctgtc aaacatgaga attgacccgg 
    4751  aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc 
    4801  cctcgactac gtcgttaagg ccgtttctga cagagtaaaa ttcttgaggg 
    4851  aactttcacc attatgggaa atggttcaag aaggtattga cttaaactcc 
    4901  atcaaatggt caggtcattg agtgttttta atttgttgta tttttttttt 
    4951  ttagagaaaa tcctccaata tataaattag gaatcatagt ttcatgattt 
    5001  tctgttacac ctaacttttt gtgtggtgcc ctcctccttg tcaatattaa 
    5051  tgttaaagtg caattctttt tccttatcac gttgagccat tagtatcaat 
    5101  ttgcttacct gtattccttt acatcctcct ttttctcctt cttgataaat 
    5151  gtatgtagat tgcgtatata gtttcgtcta ccctatgaac atattccatt 
    5201  ttgtaatttc gtgtcgtttc tattatgaat ttcatttata aagtttatgt 
    5251  acaaatatca taaaaaaaga gaatcttttt aagcaaggat tttcttaact 
    5301  tcttcggcga cagcatcacc gacttcggtg gtactgttgg aaccacctaa 
    5351  atcaccagtt ctgatacctg catccaaaac ctttttaact gcatcttcaa 
    5401  tggccttacc ttcttcaggc aagttcaatg acaatttcaa catcattgca 
    5451  gcagacaaga tagtggcgat agggttgacc ttattctttg gcaaatctgg 
    5501  agcagaaccg tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg 
    5551  gcaaagaggc caaggacgca gatggcaaca aacccaagga acctgggata 
    5601  acggaggctt catcggagat gatatcacca aacatgttgc tggtgattat 
    5651  aataccattt aggtgggttg ggttcttaac taggatcatg gcggcagaat 
    5701  caatcaattg atgttgaacc ttcaatgtag ggaattcgtt cttgatggtt 
    5751  tcctccacag tttttctcca taatcttgaa gaggccaaaa cattagcttt 
    5801  atccaaggac caaataggca atggtggctc atgttgtagg gccatgaaag 
    5851  cggccattct tgtgattctt tgcacttctg gaacggtgta ttgttcacta 
    5901  tcccaagcga caccatcacc atcgtcttcc tttctcttac caaagtaaat 
    5951  acctcccact aattctctga caacaacgaa gtcagtacct ttagcaaatt 
    6001  gtggcttgat tggagataag tctaaaagag agtcggatgc aaagttacat 
    6051  ggtcttaagt tggcgtacaa ttgaagttct ttacggattt ttagtaaacc 
    6101  ttgttcaggt ctaacactac cggtaccgcg cttgcggaag catcagcaaa 
    6151  taaggccagc acagccagcg cagttgccgc tttggttcct gattctgttc 
    6201  ttgatgaatt aaacaaagcg gcacagtaac aaaggacttc attgataatt 
    6251  tttcttcagg aggaagacat gtcattcttt tctacgttaa aaacagcttt 
    6301  gtctttgaag gagaaacttg ctgctactgg tgttcttgtt ctgatttgcg 
    6351  cacttgttgg tgctgggttt gcatgggaac gtcatcagct aaagcaagcc 
    6401  atagagaaaa ttggcagtct tgatcaggct gttaaggaac gtgataagtc 
    6451  aataatggat cttaaccaga ccattgagac gatgaacaaa gcagagcaac 
    6501  attttcacag ccaggaagtg aaaaatgaat cagaacaagc caagtatgct 
    6551  gacaggcaaa tggaacgaaa agctgaagtt cagaaacaac tggttgcggc 
    6601  gggtaatgtt cgccagcgta ttcctgctga cactcagcgg ttgctccggg 
    6651  agtcgatcag cgaatttaac gccgacgccg acaaaggtta accaccctgc 
    6701  ccccaaaagt gcatttatgt gtaggatgcc agagtttagc agtgaatatt 
    6751  ttgatgatct gccagcgtat atcctcgata cagaaacgat gctgatgggg 
    6801  attaacagga agaatcgcaa cgttaatgat tacaaccgag ctattagcgg 
    6851  taactaaaag ggatttttat gtctgataaa gtaacagtaa agcaaactat 
    6901  caacaaagcg acttcaatct acaaaattga gcaaatcact gttggcaagc 
    6951  caggatctga acaataccgt cgtgctttcg agcttgccga tcagcttggt 
    7001  ttaaaacacc cggattgcat tgagcatgta tttccgacct atgctgatga 
    7051  gcaatgtact catgttctta ccgaagagga ttttttcagc actgaagaac 
    7101  gagaaggcgt tgatcgctgc attggtgtga tttgttcttc ggtaagtgat 
    7151  gagttattcc ctaatgtgcc tgaatatggt ggtattggat accaattcct 
    7201  gtacgagggc gatgagctta aatgctatga acatggtctt ctcatcgaaa 
    7251  gcgtagaata atacgactcc cttccaaccg gctacgttgg ccggtttttc 
    7301  acttatccac attatccact ggatagatcc aataatcagg tccatacaga 
    7351  tcccaattag atccatatag atccctgatc gttgcaggcc gcgccacgtc 
    7401  tggcttagaa gtgtatcgcg atgtgtgctg gagggaaaac gatgtgtgct 
    7451  ggagggataa aaatgtgtgc tgacgggttg ctaatgtgtg ctggcgggat 
    7501  ataggatgtg tgttgacggg aaagcttggg tagttatcac cacttataaa 
    7551  aactatccac acaattcgga aaaagtaata tgaatcaatc atttatctcc 
    7601  gatattcttt acgcagacat tgaaagtaag gcaaaagaac taacagttaa 
    7651  ttcaaacaac actgtgcagc ctgtagcgtt gatgcgcttg ggggtattcg 
    7701  tgccgaagcc atcaaagagc aaaggagaaa gtaaagagat tgatgccacc 
    7751  aaagcgtttt cccagctgga gatagctaaa gccgagggtt acgatgatat 
    7801  taaaatcacc ggtcctcgac tcgatatgga tactgatttc aaaacgtgga 
    7851  tcggtgtcat ctacgcgttc agcaaatacg gcttgtcctc aaacaccatc 
    7901  cagttatcgt ttcaggaatt cgctaaagcc tgtggtttcc cctcaaaacg 
    7951  tctggatgcg aaactgcgtt taaccattca tgaatcactt ggacgcttgc 
    8001  gtaacaaggg tatcgctttt aagcgcggaa aagatgctaa aggcggctat 
    8051  cagactggtc tgctgaaggt cgggcgtttt gatgctgacc ttgatctgat 
    8101  agagctggag ggctgattcg aagttgtggg agctgttcca gcttgattat 
    8151  cgcgttctgt tgcaacacca cgccttgcgt gcccttccga agaaagaagc 
    8201  tgcacaagcc atttacactt tcatcgaaag ccttccgcag aacccgttgc 
    8251  cgctatcgtt cgcgcgaatc cgtgagcgcc tggctttgca gtcagctgtt 
    8301  ggcgagcaaa accgtatcat taagaaagcg atagaacagc ttaaaacaat 
    8351  cggctatctc gactgttcta ttgagaagaa aggccgggaa agttttgtaa 
    8401  tcgtccattc tcgcaatcca aagctgaaac tccccgaata agtgtgtgct 
    8451  ggagggaaac cgcattaaaa agatgtgtgc tgccgggaag gcttgtccaa 
    8501  tttcctgttt ttgaatgtgc gctggagggg gacgcccctc agtttgccca 
    8551  gactttccct ccagcacaca tctgtccatc cgcttttccc tccagtgcac 
    8601  atgtaattct ctgcctttcc ctccagcaca catatttgat accagcgatc 
    8651  cctccacagc acataattca atgcgacttc cctctatcgc acatcttaga 
    8701  cttttattct ccctccagca cacatcgaag ctgccgggca agccgttctc 
    8751  accagttgat agagagtgaa gcttggctgc ccattgaagc aggaaatcac 
    8801  caaaatgatt caggctacaa cctgaacgta gaagaaatcc gcgtccttta 
    8851  tgcgtggagg atgccaaagc atgttgtgac acacttggca aaggagtaag 
    8901  catgcagaga atgctatgta caagcatcta cgcatacatt attattttat 
    8951  gcagcatttt taattaaatt caaaaataca gcataaagga tgactttcga 
    9001  tgagtgattc cagccagctt cacaaggttg ctcaaagagc aaacagaatg 
    9051  ctcaatgttc tgactgaaca agtacagttg caaaaggatg agctacacgc 
    9101  gaacgagttt taccaggtct atgcgaaagc ggcactggca aaattgcctc 
    9151  tactgactcg agcgaacgtt gactatgccg taagtgaaat ggaagaaaag 
    9201  ggttatgttt tcgataaacg ccctgctggc tcttcaatga aatatgcgat 
    9251  gtcaattcag aacatcattg acatatatga acatcgcgga gtgccaaaat 
    9301  accgggatcg ctacagcgaa cgtatgtgat tttcatctcc aatcttaaag 
    9351  gcggtgtgtc aaaaactgta tcgacggttt ctctggcgca tgcaatgcgt 
    9401  gctcaccctc atcttcttat ggaggattta aggattctgg ttattgacct 
    9451  tgatccgcaa tcttcagcaa cgatgttttt aagccataaa cactctattg 
    9501  gtatcgtaaa cgcaacatct gcacaggcta tgttgcagaa tgtaagccgt 
    9551  gaagagctgt tagaggagtt tattgttcct tctgttgtac ctggggttga 
    9601  cgttatgcct gcgtcgattg acgatgcctt tattgcatcc gattggagag 
    9651  agctgtgcaa tgagcatcta ccgggtcaga acatccatgc tgtcctgaaa 
    9701  gaaaatgtga ttgataagct gaagagcgat tatgacttta tcctcgttga 
    9751  tagtggtcct caccttgacg ccttcctgaa aaatgctttg gcctcggcca 
    9801  atatactgtt tacacctctg ccgccagcaa ctgtcgattt ccactcatcg 
    9851  cttaaatacg ttgcccgcct tcctgagttg gtgaactcat ttcggatgaa 
    9901  ggctgcgagt gccagcttgc gactaacatt ggttttatgt ccaagttgag 
    9951  taacaaggca gaccataagt attgccatag cctggctaaa gaagtgttcg 
   10001  gtggggatat gcttgatgtc ttcctccctc gccttgacgg ttttgaaacg 
   10051  ctgcggcgag tcttttgaca ctgttatttc agctaacccg gcaacgtatg 
   10101  ttggtagtgc tgatgcattg aagaacgcgc gaattgccgc ggaagatttt 
   10151  gctaaagcag tttttgaccg tattgaattt atcagatcta actgaggagt 
   10201  aagaaacccc catgtcaaag aaaaacagac caacaattgg gcgaaccctt 
   10251  aatccttcaa tattaagcgg atttgatagt tcttcagcct ctggcgatcg 
   10301  agtcgagcag gtattcaagt tatcaactgg tcgccaggcc acatttattg 
   10351  aagaggtaat acctccgaac caggtagaaa gcgatacctt tgttgatcag 
   10401  cataacaacg ggcgtgacca ggcatctctt acgccaaaat cattaaaaag 
   10451  tatccgaagc actattaagc atcagcaatt ttaccctgca ataggtgtta 
   10501  gacgggctac agggaaaatt gaaattttgg atggttcccg gcgtcgagct 
   10551  tctgccatct tagagaacgt agggttgcgg gttttagtca cggaccagga 
   10601  gatcagcgtt caggaagcgc aaaatttagc gaaagacgtt cagacagcat 
   10651  tgcagcacag cattcgagaa ataggtctgc gtttgatgcg aatgaaaaat 
   10701  gatgggatga gtcagaagga tattgcagcc aaagaagggc tgtctcaggc 
   10751  gaaggtcacg cgtgctctcc aggcagcgag tgctccggaa gaattagtcg 
   10801  cccttttccc tgtgcagtcg gaattaacct tttcggacta caaaacgctt 
   10851  tgtgctgttg gcgacgaaat ggggtacaag aatttagagt ttgatcagct 
   10901  tattcaaaac atatccccgg aaataaacga catcttatcc attgaagaaa 
   10951  tggccgaaga tgaagttaaa aataaaatcc tgcgcttgat aacaaaggaa 
   11001  gcctcactac tcacggataa aggttctaaa gataagtccg tagttactga 
   11051  attatggaaa tttgaggaca aggatcgctt tgcaaggaag cgcgtgaaag 
   11101  gccgtgcatt ttcttatgag tttaatcgac tctcaaaaga gttacaggaa 
   11151  gaactcgaca ggatgattgg gcatatcctt agaaagagcc tcgataaaaa 
   11201  gccgaagcct taaactttcg ccattcaaat ttcactatta actgactgtt 
   11251  tttaaagtaa attactctaa aatttcaagg tgaaatcgcc acgatttcac 
   11301  cttggatttt accttcctcc cctcctcccg aaaaaaataa aaaaattgct 
   11351  tgtcacgaga aagtcaacaa gtgactttca ataaaatctc ttccgaaaag 
   11401  ggattcacac aagtgccttg tgtttaagga agagtaaatt gagtaactta 
   11451  cgcgaatacc agaatcgtat tgcagatatc gcaaaacgct ctaaagctgt 
   11501  gcttggctgg gcaagcactg cgcagttcgg tactgataac caattcatta 
   11551  aagatgatgc cgcgcgtgcc gcatctatcc ttgaagctgc acgtaaagac 
   11601  ccggtttttg cgggtatctc tgataatgcc accgctcaaa tcgctacagc 
   11651  gtgggcaagt gcactggctg actacgccgc agcacataaa tctatgccgc 
   11701  gtccggaaat tctggcctcc tgccaccaga cgctggaaaa ctgcctgata 
   11751  gagtccaccc gcaatagcat ggatgccact aataaagcga tgctggaatc 
   11801  tgtcgcagca gagatgatga gcgtttctga cggtgttatg cgtctgcctt 
   11851  tattcctcgc gatgatcctg cctgttcagt tgggggcagc taccgctgat 
   11901  gcgtgtacct tcattccggt tacgcgtgac cagtccgaca tctatgaagt 
   11951  ctttaacgtg gcaggttcat cttttggttc ttatgctgct ggtgatgttc 
   12001  tggacatgca atccgtcggt gtgtacagcc agttacgtcg ccgctatgtg 
   12051  ctggtggcaa gctccgatgg caccagcaaa accgcaacct tcaagatgga 
   12101  agacttcgaa ggccagaatg taccaatccg aaaaggtcgc actaacatct 
   12151  acgttaaccg tattaagtct gttgttgata acggttccgg cagcctactt 
   12201  cactcgttta ctaatgctgc tggtgagcaa atcactgtta cctgctctct 
   12251  gaactacaac attggtcaga ttgccctgtc gttctccaaa gcgccggata 
   12301  aaggcactga gatcgcaatt gagacggaaa tcaatattga agccgctcct 
   12351  gagctgatcc cgctgatcaa ccacgaaatg aagaaataca ccctgttccc 
   12401  aagtcagttc gttatcgcgg ctgagcacac ggtacaggcg gcgtatgaag 
   12451  cacagcgtga atttggtctg gacctgggtt ccctacagtt ccgcaccctg 
   12501  aaggaatacc tgtctcatga acaggatatg ctgcgtcttc gcatcatgat 
   12551  ctggcgcact cttgcgaccg acacctttga catcgctctg ccggttaacc 
   12601  agtcctttga tgtatgggca accatcattc gtggcaaatt ccagactgta 
   12651  tatcgcgaca ttattgagcg cgttaaatct tctggtgcga tggggatgtt 
   12701  tgctggtgct gatgcagcat ctttcttcaa acagttgccg aaggatttct 
   12751  tccagccagc cgaagactat atccagactc cgtatgttca ctacatcggt 
   12801  accccattta ggaccaccca cagcacctaa caaaacggca tcagccttct 
   12851  tggaggcttc cagcgcctca tctggaagtg gaacacctgt agcatcgacc 
   12901  tgcagggggg ggggggcgct gaggtctgcc tcgtgaagaa ggtgttgctg 
   12951  actcatacca ggcctgaatc gccccatcat ccagccagaa agtgagggag 
   13001  ccacggttga tgagagcttt gttgtaggtg gaccagttgg tgattttgaa 
   13051  cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct 
   13101  gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccgccgtccc 
   13151  gtcaagtcag cgtaatgctc tgccagtgtt acaaccaatt aaccaattct 
   13201  gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc 
   13251  aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag 
   13301  aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct 
   13351  gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc 
   13401  aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg 
   13451  gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc 
   13501  cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt 
   13551  cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag 
   13601  gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc 
   13651  gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa 
   13701  tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag 
   13751  tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag 
   13801  tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc 
   13851  atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga 
   13901  ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat 
   13951  aaatcagcat ccatgttgga atttaatcgc ggcctcgagc aagacgtttc 
   14001  ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag 
   14051  acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat 
   14101  cagagatttt gagacacaac gtggctttcc cccccccccc tgcaggtcga 
   14151  tagcagcacc accaattaaa tgattttcga aatcgaactt gacattggaa 
   14201  cgaacatcag aaatagcttt aagaacctta atggcttcgg ctgtgatttc 
   14251  ttgaccaacg tggtcacctg gcaaaacgac gatcttctta ggggcagaca 
   14301  ttagaatggt atatccttga aatatatata tatattgctg aaatgtaaaa 
   14351  ggtaagaaaa gttagaaagt aagacgattg ctaaccacct attggaaaaa 
   14401  acaataggtc cttaaataat attgtcaact tcaagtattg tgatgcaagc 
   14451  atttagtcat gaacgcttct ctattctata tgaaaagccg gttccggcgc 
   14501  tctcaccttt cctttttctc ccaatttttc agttgaaaaa ggtatatgcg 
   14551  tcaggcgacc tctgaaatta acaaaaaatt tccagtcatc gaatttgatt 
   14601  ctgtgcgata gcgcccctgt gtgttctcgt tatgttgagg aaaaaaataa 
   14651  tggttgctaa gagattcgaa ctcttgcatc ttacgatacc tgagtattcc 
   14701  cacagttaac tgcggtcaag atatttcttg aatcaggcgc cttagaccgc 
   14751  tcggccaaac aaccaattac ttgttgagaa atagagtata attatcctat 
   14801  aaatataacg tttttgaaca cacatgaaca aggaagtaca ggacaattga 
   14851  ttttgaagag aatgtggatt ttgatgtaat tgttgggatt ccatttttaa 
   14901  taaggcaata atattaggta tgtagatata ctagaagttc tcctcgacgc 
   14951  tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag 
   15001  gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc 
   15051  ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa 
   15101  gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc 
   15151  ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca 
   15201  cgatgcgtcc ggcgtagagg atctacaact ccacttattg ttaggtagaa 
   15251  ttgtccgtta gttgtttatt aattgcaata atggggcgtc cagttttggc 
   15301  aacagtgtcc tcttaccagg acacctatga gtttgcctca tggcaaacta 
   15351  gaggtgttga aagtatgcat ggttataatt agagcaattc attaccctct 
   15401  gaatcctgcc ggtatacccc attgttcgtt atctttattt ttggctaaaa 
   15451  ccgcattaag agcttcgttt accgtcatgc aatgcggtag gttatcgaag 
   15501  tttgatatcc cgccaatatc aggcgaacgc ttgttcttca ggtaagcata 
   15551  tttccgcgca gcgcctctac tttctgcttg aactcatgtt tttgagtgcg 
   15601  ttttttggat aaccgcagat tgtcagcctt tgcttttgcc ttagcgatcc 
   15651  atgaagtcaa ttttttgagg ctggttgttc cggcaccgcc ggaaactgat 
   15701  ctttttgttt ttttaacttg tgacttctta ttctttattg ccacgtcatc 
   15751  ctgacagggg gagggggtat cattttgaca tgggggtgtg gataaaaaat 
   15801  taaataaagc caatgtctta gcgagaacag ctttaacctt ggttgccgct 
   15851  gaagagatct ttaatttgct ttctatcagc gcatttttgg cttgttgtgc 
   15901  gaaggccaaa aaggatggtg taaaccggta caggttagcg cgacgttcac 
   15951  ggtgatcgcc gataacaatc tctacagaca gaattccttt gtttacagct 
   16001  tcacggaatg cacgaacgac ggttgattgg ctataaccag tttctgccgc 
   16051  gatcaggcgg tgaggcttgt gaatgaagta ttcactggtt gttgccgcga 
   16101  gatttgcaca ttgcgacagg atatgcccgg cgctacggga tagaccggag 
   16151  tgtgttacaa agcaggccaa ttcatagcca gaaaaagtaa aatcgctcat 
   16201  cgttatacag ctcaggaaag tgactttagc cagcattaca atgctggtgg 
   16251  ttcttactac gtctgttagc gttgccgcga caggtaccag cacaccagca 
   16301  tcaagcaatc gcttcatcag ccactgctga cctttgccgg ttatacgagt 
   16351  cgtgaaagaa atcctgcttc cattgcttgt atcgatcacg gtttctttaa 
   16401  gggtgaaata cccacgagat atgtattctt gtttggggac gttcctgcgt 
   16451  tcaccggttg cgatcagaat tccgttatca cgcaaccagg tgaagagata 
   16501  gttttggccc aggccgagca ctttggcata gttgccgatt agaaccccgc 
   16551  tggcggtagc aacgcgttca gcgaattcga ctttaggtgc atccataagc 
   16601  attttttgct ccagccgttg cttttgctct gccaggtcgg cagccaaacg 
   16651  gagagcttca gggagactct gcggaatagc aggttgtaat cttccggttc 
   16701  gatagtcgat aaatgtctgg tttagctttc agcccgaaac gcgggagaaa 
   16751  tccagcctgc gtactcccac agcgaagcaa tttcattggg gcaaaagttg 
   16801  ccgccggcaa gggcctttcg gacctgcagg cattgcaagc ttggcgtaaa 
   16851  tccatgggtc atagctggtt cccgtgtgtg aaaattgtta ccggttcaca 
   16901  attccacaca acatacgagc cggaagcata aagtgtaaag ccttgggttg 
   16951  cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 
   17001  ttccagtcgg gaaacctgtc gtgccaggta gtcgatatgg tgcactctca 
   17051  gtacaatctg ctctgatgcc gcatagttaa gccagtatac actgccgtat 
   17101  cgctacgtga ctgggtcatg ctgcgccccg acacccgcca acatcccgtg 
   17151  aacagcctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 
   17201  tgaccgtctc cgggagctgc atgtgtcaga gttttcaccg tcatcaccga 
   17251  aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat 
   17301  tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 
   17351  aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt 
   17401  tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg 
   17451  ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac 
   17501  tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg 
   17551  cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag 
   17601  cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 
   17651  tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt 
   17701  ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag 
   17751  gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg 
   17801  tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc 
   17851  tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg 
   17901  cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat 
   17951  gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat 
   18001  tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt 
   18051  ccattcaggt cgaggtggcc cggctccatg caccgcgacg caaacgcggg 
   18101  gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc 
   18151  atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccaatg 
   18201  atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg 
   18251  atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc 
   18301  cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct 
   18351  cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc 
   18401  ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga 
   18451  cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg 
   18501  ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca 
   18551  gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca 
   18601  taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact 
   18651  gggttgaagg ctctcaaggg catcggtcga gcttgacatt gtaggactat 
   18701  attgctctaa taaatttgcg gccgctaata cgactcacta tagggagagg 
   18751  atcg

//
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