Sanger Sequencing Client Portal

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Start Sequencing Submission

Sanger Sequencing Client Portal

Purchase eVouchers

Start Sequencing Submission

How It Works

Technical Specifications Workflow

Sample QC of incoming extracted DNA and RNA is analysed to determine the quality of the fragments and the quantity of the sample. You will be provided with an initial QC report with recommendations on the suitability of your samples for project progression.
Library Preparation: The selection of the library preparation is made depending on the type of sample and sequencing being undertaken. Supplier protocols are followed for the library preparation to ensure the quality of the library for sequencing.
Library Validation: For routine library validation, another rigorous quality control is performed to ascertain the library fragment size, yield and concentration.
Cluster Generation: During cluster generation, the library hybridises to the flow cell and a series of bridge amplifications occur.
Sequencing by Synthesis: This process involves the addition of fluorescently labelled reversible-terminator nucleotides which compete to bind the strand tethered to the flow cell. Once the correct base binds, laser excitation is used to emit a wavelength, and this specific wavelength is detected by a high-resolution camera. Once this has been detected, the fluorescent and terminator sequences of the nucleotide are cleaved, allowing the addition of a new fluorescent nucleotide. This occurs in a “massively parallel” fashion to obtain large amounts of sequencing data.
Lab QC report detailing the integrity of the samples that were used for sequencing.
Bioinformatics pipeline report, explaining quality control metrics used to assess the quality of the data, and details of all bioinformatics analyses that have been performed, explaining the methods used and results obtained.