Below is listed the optimal sample requirements needed. The stated volumes provide us with enough of the sample for our internal sample checks and library preparation. Samples can be provided more concentrated than suggested and will be diluted as needed. Please contact your Sales representative for minimum sample requirements. DNA Library preparation Optimal input quantity Optimal volume required (μl) Optimal concentration (ng/μl) Standard gDNA 500ng 50 10 Low input gDNA 100ng 20 5 Ultra low input gDNA 2ng 50 40pg/μl PCR-free gDNA 3ug 50 60 Exome/ Custom Enrichment Library preparation Optimal input quantity Optimal volume required (μl) Optimal concentration (ng/μl) Agilent SureSelect All Human Exon V7 1000ng 50 20 IDT 1000ng 50 20 Twist BioScience Human Core Exome 1000ng 50 20 RNA Library preparation Optimal input quantity Optimal volume required (μl) Optimal concentration (ng/μl) Standard stranded cDNA (polyA selection) 300ng 50 6 Standard stranded cDNA (rRNA depletion) 300ng 50 6 Low input cDNA (polyA selection) 50ng 50 1pg/μl Low input cDNA (polyA selection) 50ng 50 1pg/μl Amplicon / 16S / Index PCR Library preparation Optimal input quality Optimal volume required (μl) Optimal concentration (ng/μl) Target amplification 100ng 10 10ng/μl Ready to sequence pool requirements Sequencing instrument Minimum volume required Min Concentration (nM/μl) MiSeq 20 15 NextSeq 20 15 NovaSeq SP 100 6 NovaSeq S1 100 6 NovaSeq S2 150 6 NovaSeq S4 310 6 Amplicon 16S/18S/ITS Sample type Amount Volume Purity Genomic DNA ≥ 200ng ≥ 40μL OD260/280=1.8-2.0 Shotgun metagenomics Samples sources including faeces, natural environments, as well as DNA samples Sample Type Amount Volume Purity Genomic DNA ≥ 200ng ≥ 40μL OD260/280=1.8-2.0