Below is listed the optimal sample requirements needed.  The stated volumes provide us with enough of the sample for our internal sample checks and library preparation.  Samples can be provided more concentrated than suggested and will be diluted as needed. Please contact your Sales representative for minimum sample requirements.

DNA

Library preparation Optimal input quantity Optimal volume required (μl) Optimal concentration (ng/μl)
Standard gDNA 500ng 50 10
Low input gDNA 100ng 20 5
Ultra low input gDNA 2ng 50 40pg/μl
PCR-free gDNA 3ug 50 60

 

Exome/ Custom Enrichment

Library preparation Optimal input quantity Optimal volume required (μl) Optimal concentration (ng/μl)
Agilent SureSelect All Human Exon V7 1000ng 50 20
IDT 1000ng 50 20
Twist BioScience Human Core Exome 1000ng 50 20

 

RNA

Library preparation Optimal input quantity Optimal volume required (μl) Optimal concentration (ng/μl)
Standard stranded cDNA (polyA selection) 300ng 50 6
Standard stranded cDNA (rRNA depletion) 300ng 50 6
Low input cDNA (polyA selection) 50ng 50 1pg/μl
Low input cDNA (polyA selection) 50ng 50 1pg/μl

 

Amplicon / 16S / Index PCR

Library preparation Optimal input quality Optimal volume required (μl) Optimal concentration (ng/μl) 
Target amplification 100ng 10 10ng/μl

 

Ready to sequence pool requirements

Sequencing instrument Minimum volume required Min Concentration (nM/μl)
MiSeq 20 15
NextSeq 20 15
NovaSeq SP 100 6
NovaSeq S1 100 6
NovaSeq S2 150 6
NovaSeq S4 310 6

 

Amplicon 16S/18S/ITS 

Sample type Amount Volume Purity
Genomic DNA ≥ 200ng ≥ 40μL OD260/280=1.8-2.0

Shotgun metagenomics

Samples sources including faeces, natural environments, as well as DNA samples

Sample Type Amount Volume Purity
Genomic DNA ≥ 200ng ≥ 40μL OD260/280=1.8-2.0