Prepare Your Samples
Enter details of the sample submission format including tubes, plates and orientation of the plate.
Primer Synthesis (optional add-on)
Source BioScience stocks a collection of 40 common primers from which you can choose (free of charge) for your Sanger Sequencing. If, however, you require a primer/s that we do not stock, you might like to make use of our Primer Synthesis option.
Secondary Structure Resolution (optional add-on)
When sequencing templates which are G-C-rich, contain hairpin structures, repetitive regions or troublesome sequences, we recommend including our Secondary Structure Treatment Solution (dGTP chemistry) with your order.
This optional add-on aids the sequencing process by disrupting the production of secondary structures, as well as aiding the initiation of the sequencing process, to yield higher quality reads.
Plasmid DNA to be supplied at a concentration of 100ng/ul in a volume of 5ul per reaction.
Sequencing primers to be sent at a concentration of 3.2pmol/ul in a volume of 5ul per reaction.