MGC (Mammalian Gene Collection)

The Mammalian Gene Collection (MGC) project is an NIH initiative. The goal of the project was to make available a complete set of full-length cDNA clones from human, rat, mouse and cow.

MGC cDNA clones were obtained by screening of cDNA libraries, by transcript-specific RT-PCR cloning, and by DNA synthesis of cDNA inserts

 

  • 969_IRAU  IRAU MGC Human verified full length cam cDNA
  • 970_IRAT  IRAT MGC Human verified full length amp cDNA
  • 5012_IRCM IRCM MGC Human verified full length kan cDNA
  • 5020_IRCV IRCV MGC Human synthesized DNA
  • 968_IRAV IRAV MGC Mouse verified full length amp cDNA
  • 5000_IRAW IRAW MGC Mouse verified full-length cam cDNA
  • 5011_IRCL IRCL MGC Mouse verified full length kan cDNA
  • 5021_IRCW IRCW MGC Mouse synthesized DNA
  • 993_IRBP IRBP MGC Rat verified full length amp cDNA
  • 994_IRBQ IRBQ MGC Rat verified full length cam cDNA
  • 5010_IRCJ IRCJ MGC Cow verified full length amp cDNA

Protocols / Clone Handling

Clones should be stored at +4°C on receipt. As soon as possible, re-streak the clones onto the same medium and incubate overnight at 37°C to obtain single colonies.

A selection of at least 10 single colonies should be picked and grown in LB broth containing the appropriate antibiotic +8 % glycerol for subsequent freezing at -70°C.

These frozen stocks can then be re-grown for plasmid isolation and purification.

References

Strausberg RL, Feingold EA, Klausner RD & Collins FS(1999). The Mammalian Gene Collection. Science 286: 455-457.

MGC (Mammalian Gene Collection) Program Team (2002). Generation and Initial Analysis of more than 15000 Full-Length Human and Mouse cDNA sequences. PNAS 99(26): 16899-16903.


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