XGC (Xenopus Gene Collection)
The Xenopus Gene Collection (XGC) project is trans-NIH initiative, which was launched, as a subproject of the existing Mammalian Gene Collection. The general approach is to acquire or construct high-quality cDNA libraries. The libraries are screened by sequencing the 5' end of the clones. Putative full open reading frames clones are identified, and finally, selected clones are full-length sequence verified. The goal of the project was to make available a complete set of these full-length cDNA clones in Xenopus laevisandXenopus tropicalis.
IRBG XGC X. laevis verified full-length cam cDNA 6 96 576
IRBN XGC X. tropicalis full length amp cDNA 40 96 3,840
IRBH XGC X. laevis full length amp cDNA 96 96 9,216
IRBJ MGC Xenopus full length cDNA 7 384 1,999
These clones are for research use only
- Easy generation of clones suitable for protein expression in various systems via Gateway shuttle reaction
- Expression of native proteins or fusion proteins including N.- or C-terminal tags
- Investigation of protein structure and function
- Protein localisation studies
- In vitro transcription
- RNAi rescue
For individual XGC cDNA clone orders please use our website search engine
Search using accession number, gene ID or IMAGE_ID (you cannot use the MGC_ID)
Clones are provided without restrictions to their use in academia and industry, except for the terms specified in a Good Faith Agreement and our clone terms and conditions.
1. Please check the GenBank record of each MGC full-length clone for detailed sequence annotation. The definition of what constitutes a full-length coding region for some of the genes and transcripts of MGC clones may have changed since the clones were produced and you should carefully check the clone insert sequence shown on NCBI to make sure that it matches the sequence you expect.
2. To overcome problems associated to wrong clone identities in large public resources, Source BioScience performs an additional clone verification by Sanger end sequencing reads (5' and/or 3'). Our sequence verification consists of a quality clipped, but otherwise uncurated end reads (and not full-length-sequencing). The result will be compared to the sequences already published for the particular clone and the resulting alignment confirms the clone identity. Using this service, only clones matching the clone reference sequence will be delivered and charged. Clones which cannot be confirmed will not be charged.
Source BioScience assumes no liability for clone orders where the client chooses to reject the offered end sequence verification option.
The use of the resource is limited to research purposes. The collection is subject to the following MTA.
IRBH X. laevis Vectors confer ampicillin resistance
IRBG X. laevis Vectors confer chloramphenicol resistance
IRBN X. tropicalis Vectors confer ampicillin resistance
Other technical details for each clone can be found on our website search, along with the background vector and link to the sequence accession number.
Protocols / Clone Handling
Clones should be stored at +4°C on receipt. As soon as possible, re-streak the clones onto the same medium and incubate overnight at 37°C to obtain single colonies.
A selection of at least 10 single colonies should be picked and grown in LB broth containing the appropriate antibiotic and 8 % glycerol for subsequent freezing at -70°C.
These frozen stocks can then be re-grown for plasmid isolation and purification.
R. D. Morin et al., Sequencing and analysis of 10,967 full-length cDNA clones from Xenopus laevis and Xenopus tropicalis reveals post-tetraploidization transcriptome remodeling. Genome Res. 16(6), 796-803 (June 2006).
MGC Project Team, The status, quality, and expansion of the NIH Full-length cDNA Project: The Mammalian Gene Collection (MGC). Genome Res. 14(10B), 2121-2127 (October 2004).
R. L. Strausberg et al., The Mammalian Gene Collection. Science. 286(5439), 455-457 (15 October 1999).
For further information and prices please contact us or call +44 (0)115 973 9012