ZGC (Zebrafish Gene Collection)
The Zebrafish Gene Collection (ZGC) project is an NIH initiative which was launched in April 2002, as a subproject of the existing Mammalian Gene Collection. The general approach is to acquire or construct high-quality cDNA libraries. The libraries are screened by sequencing the 5' end of the clones. Putative full open reading frames are identified, and finally, selected clones are full-length sequence verified. The goal of the project is to make available a complete set of these full-length cDNA clones in Zebrafish.
IRBO ZGC Zebrafish full length amp cDNA
IRCX Zebrafish predicted full length kan cDNA
IRCY Zebrafish PCR rescued genes kan cDNA
IRBV ZGC Zebrafish verified full-length cam cDNA
Clones from this subset can be ordered as individual clones (streaks on agar) and 96-well plates (frozen bacterial stocks in glycerol).
Search using accession number, gene ID or IMAGE_ID (you cannot use the MGC_ID)
The entire sets can be ordered below:
| Product Code |
Name |
Price |
| 991_IRBO |
IRBO ZGC Zebrafish full length amp cDNA |
£9,089.00
|
| Product Code |
Name |
Price |
| 5022_IRCX |
IRCX Zebrafish predicted full length kan cDNA |
£271.00
|
| Product Code |
Name |
Price |
| 5006_IRBV |
IRBV ZGC Zebrafish verified full-length cam cDNA |
£1,271.00
|
| Product Code |
Name |
Price |
| 5023_IRCY |
IRCY Zebrafish PCR rescued genes kan cDNA |
£2,044.00
|
Clones are provided without restrictions to their use in academia and industry, except for the terms specified in a Good Faith Agreement and our clone terms and conditions.
Please note:
1. Please check the GenBank record of each ZGC full-length clone for detailed sequence annotation. The definition of what constitutes a full-length coding region for some of the genes and transcripts of MGC clones may have changed since the clones were produced and you should carefully check the clone insert sequence shown on NCBI to make sure that it matches the sequence you expect.
2. To overcome problems associated to wrong clone identities in large public resources, Source BioScience performs an additional clone verification by Sanger end sequencing reads (5' and/or 3'). Our sequence verification consists of a quality clipped, but otherwise uncurated end reads (and not full-length-sequencing). The result will be compared to the sequences already published for the particular clone and the resulting alignment confirms the clone identity. Using this service, only clones matching the clone reference sequence will be delivered and charged. Clones which cannot be confirmed will not be charged.
Source BioScience assumes no liability for clone orders where the client chooses to reject the offered end sequence verification option.
ZGC vectors
pBluescriptSK(-)
pDNR-LIB
pME18S-FL3
pCMV-SPORT6.1
pExpress-1
The technical details for each clone can be found on our website search, along with the background vector and link to the sequence accession number.
Protocols / Clone Handling
IRBO (Zebrafish)
The clones have been streaked onto LB agar containing 50µg/ml ampicillin. Please store them at 4°C (not in a freezer).
As soon as possible, re-streak the clones onto the same medium and incubate overnight at 37°C to obtain single colonies.
IRBV (Zebrafish)
The clones have been streaked onto LB agar containing 57µg/ml chloramphenicol. Please store them at 4°C (not in a freezer).
As soon as possible, re-streak the clones onto the same medium and incubate overnight at 30°C to obtain single colonies.
IRCX and IRCY (Zebrafish), kanamycin, 30 μg/ml
A selection of at least 10 single colonies should be picked and grown in LB broth containing the appropriate antibiotic + 8% glycerol for subsequent freezing at -70°C. These frozen stocks can then be re-grown for plasmid isolation and purification.
Streaking out for single colonies is essential in case of contamination or cross-contamination, or if modifications of the original clone have taken place as the library has been replicated. If there is a problem, the more single colonies you streak out, the more likely it is that you can retrieve the original construct. You can also isolate the original clone from a contaminated sample by following this procedure.
References
MGC (Mammalian Gene Collection) Program Team (2002). Generation and Initial Analysis of more than 15000 Full-Length Human and Mouse cDNA sequences. PNAS 99(26): 16899-16903
For further information and prices please contact us or call +44 (0)115 973 9012