Promoterome Resources
Following on from the success of the C. elegans ORFeome library, Marc Vidal and his lab, have given us access to distribute his C. elegans Promoterome library v1.1.
The development of systems biology requires the characterisation of expression profiles. Interpretation of such results should allow for the assessment of gene expression in cells and tissues, under different developmental stages and environmental conditions. The library includes clones containing ~6,000 predicted promoters, cloned into the highly flexible MultiSite Gateway™ system (Invitrogen). These promoters can be transferred easily into various Gateway ™ Destination vectors to drive expression of markers such as GFP alone (promoter::GFP constructs), or in fusion with protein-encoding open reading frames available in ORFeome resources (promoter::ORF::GFP).
Source BioScience offers, for research purposes only, individual clones from a single well, a subset of clones or the complete set of 71 plates (96-well format).
We can also make subsets of your chosen clones from the collection. Please contact us to discuss your requirements.
By utilising the cloned C. elegans promterome library, a large number of in vivo assays can be performed in the nematode.
- Tissue specific RNAi.
- Sub-cellular localisation, Co-immuno-precipitation and Phenotype rescue.
- Yeast-one-hybrid.ChIP-chip.
- Expression mapping.
- mRNA tagging. Blue: attB site; Red: attL site; Yellow: attR site.
Order Conditions
The use of the resource is limited to research purposes. The collection is subject to the following MTA and our clone terms and conditions.
Due to MTA requirements, individual clones and libraries should be ordered via our website.
An online C. elegans Promoter finder tool is available that allows you to search for your promoter of interest, using a C.elegans gene ID or name.
For individual clone orders please use our website search engine using the promoterome ID (wormbase). Examples of clone nomenclature are (Please note: no leading '0' in well location):
- p_T24H7.3_93
- p_Y57G11C.3_93
The entire library can be ordered below:
| Product Code |
Name |
Academic |
Commercial |
|
| 742_Cel_promoter |
C.elegans Promoterome |
£6,563.00
|
£6,563.00
|
|
We can also make subsets of your chosen clones from the collection. Please contact us to discuss your requirements.
Source Species: C elegans
Vector: pDONR P4-P1R
Protocols / Clone Handling
Please note that the clone(s) are tested as negative in our phage contamination assay prior to shipping. However, these clones should still be handled with care as no phage assay can be guaranteed to be 100% accurate.
A selection of at least 10 single colonies should be picked and grown in LB broth containing the appropriate antibiotic + 8 % glycerol for subsequent freezing at -70°C.
These frozen stocks can then be regrown for plasmid isolation and purification.
Streaking out for single colonies is essential in case of contamination or cross-contamination, or if modifications of the original clone have taken place as the library has been replicated. If there is a problem, the more single colonies you streak out, the more likely it is that you can retrieve the original construct. You can also isolate the original clone from a contaminated sample by following this procedure.
References
Genome Res. 2004 Oct;14(10B):2169-75.
"A first version of the Caenorhabditis elegans Promoterome."
Dupuy D, Li QR, Deplancke B, Boxem M, Hao T, Lamesch P, Sequerra R, Bosak S, Doucette-Stamm L, Hope IA, Hill DE, Walhout AJ, Vidal M.
Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
Acknowledgements and Subsequent Publication:
We are grateful to Dr. Marc Vidal (Dana-Farber Cancer Institute, Harvard Medical School, Boston) for the gift of this library and associated database.
Please acknowledge the Dana-Farber Cancer Institute and Source BioScience in any resulting publications, clones should be described in the paper in their ID form eg T22D1.3
Please also cite the following paper:
Dupuy D, Li QR, Deplancke B, Boxem M, Hao T, Lamesch P, Sequerra R, Bosak S,
Doucette-Stamm L, Hope IA, Hill DE, Walhout AJ, Vidal M.
“A first version of the Caenorhabditis elegans Promoterome.”
Genome Res. 2004 Oct;14(10B):2169-75.
For further information and prices please contact us or call +44 (0)115 973 9012