Human Minimal Genomic Set The Minimal Genomic Clone Set was developed on basis of NCBI's genome assembly and the Ensembl annotation. The current clone set consists of 25,130 clones. The majority (22,898 clones, 91%) of which have been completely sequenced during the human genome sequencing project. Gaps were covered by 2,232 end-sequenced clones as far as such clones were available. We focused on publicly available BAC and PAC clones and completed the set where necessary with cosmids. The overall average insert size is approx 130 kb. Product Range Applications How to order Technical Data Clone Collection in microtiter plates Customized subsets Genomic Clones are a valuable resource for the functional analysis of genes, they can be used for a multitude of applications: FISH - Fluorescent In Situ Hybridization - analysis of chromosomal rearrangements Matrix-CGH Comparative Genomic Hybridization - high resolution analysis of genomic DNA Protein - DNA binding studies - promotor, enhancer, silencer studies Subcloning of control elements Order Conditions The use of the resource is limited to research purposes. The collection is subject to the following MTA and our clone terms and conditions You can search for genomic clones by: Keywords or general IDs (like accession number, clone ID,...) using our website search below: Downloading the complete annotated clone set information as well as chromosomal subsets, listed below Human Minimal Genomic Clone Set: Chromosome 1 - 3 Human Minimal Genomic Clone Set: Chromosome 4 - 6 Human Minimal Genomic Clone Set: Chromosome 7 - 9 Human Minimal Genomic Clone Set: Chromosome 10 - 12 Human Minimal Genomic Clone Set: Chromosome 13 - 15 Human Minimal Genomic Clone Set: Chromosome 16 - 18 Human Minimal Genomic Clone Set: Chromosome 19 - 21 Human Minimal Genomic Clone Set: Chromosome 22 - XY The entire library can be ordered below: Product Code Name Price 737_HumanGenomSet Human Genomic Set - IMGS 1.0 £7,273.00 Add pBACe3.6 map and sequencepCYPAC2 map and sequencepPAC4 map and sequence Protocols / Clone Handling Individual clones have been streaked onto LB agar containing the appropriate antibiotic. Please store them at 4 °C (not in a freezer). As soon as possible, transfer the culture to LB broth containing the antibiotic + 8% glycerol and incubate overnight at 37 °C (do not try to transfer single colonies, instead scoop out the whole bacterial culture using a disposable inoculation loop). Incubate overnight at 37 °C for subsequent freezing at -70 °C. References Candidate Genes in Breast Cancer Revealed by Microarray-Based Comparative Genomic Hybridization of Archived TissueCancer Research 65, 439-447, January 15, 2005 Detection of Chromosomal Imbalances in Retinoblastoma by Matrix-Based Comparative Genomic HybridizationCANCER 43:294-301 (2005) For further information and prices please contact us or call +44 (0)115 973 9012 [email protected]