Human TilePath Genomic Set
The fully indexed Human Tilepath library, constructed at Roswell Park Cancer Institute and The Wellcome Trust Sanger Institute is a genomic clone collection of FISH mapped clones are displayed on the Ensembl genome browser.
The library was constructed as part of the Human genome project. To divide the complexity of the Human genome into reduced, workable elements, large DNA segments are cloned into either Bacterial or P1 derived artificial chromosomes.
Source BioScience offers the complete clone set of 96 384-well microtitre plates or individual clones, for research purposes only. Alternatively, the plates have been re-arrayed such that each of the chromosomes can be ordered individually.
- FISH - Fluorescent In Situ Hybridization - analysis of chromosomal rearrangements
- Matrix-CGH Comparative Genomic Hybridization - high resolution analysis of genomic DNA
- Protein - DNA binding studies - promotor, enhancer, silencer studies
- Subcloning of control elements
The library has many applications including functional studies in cell based expression assays, promoter studies and high-throughput analyses.
Due to MTA requirements, the Human Tilepath clones and library should be ordered online via our website, using the clone ID format e.g. RP11-96I16 to search for individual clones.
The use of the resource is limited to research purposes. The collection is is subject to the following MTA and our clone terms and conditions.
We recommend using the public databases to find the Human Tilepath clone you are looking for, click here for advice on how to search for your clone.
||Human TilePath Genomic Set
Protocols / Clone Handling
Individual clones have been streaked onto LB agar containing the appropriate antibiotic. Please store them at 4 °C (not in a freezer).
As soon as possible, transfer the culture to LB broth containing the antibiotic + 8% glycerol and incubate overnight at 37 °C (do not try to transfer single colonies, instead scoop out the whole bacterial culture using a disposable inoculation loop).
Incubate overnight at 37 °C for subsequent freezing at -70 °C.
Plates contain LB broth with 8% glycerol and the appropriate antibiotic. The plates are sent on Dry Ice, and should be stored at -70 °C.
Acknowledgements and subsequent publication:
Osoegawa K, Mammoser AG, Wu C, Frengen E, Zeng C, Catanese JJ, de Jong PJ. A bacterial artificial chromosome library for sequencing the complete human genome. Genome Res. 2001 Mar;11(3):483-96.
The Wellcome Trust Sanger Institute, Hixton Campus, Cambridge and Roswell Park Cancer Institute, Buffalo, New York.
For further information and prices please contact us or call +44 (0)115 973 9012