Human Expression Clones (SGC)
The Structural Genomics Consortium (SGC) provides a unique resource of verified Expression Clones which are suitable for generating well characterised and functionally active proteins or protein domains for various purposes.
Please note the SGC clones cannot currently be ordered via our website, please see below for details on how to order.
The Structural Genomics Consortium is a not-for-profit organisation set up to promote biomedical research by determining the structures of human proteins with potential therapeutic importance. Furthermore, the SGC investigates proteins from human parasites (mainly apicomplexan proteins). Thus the targets are selected based on their potential as drug targets or involvement in disease processes. The availability of high resolution structures constitutes the foundation for structure-based drug design projects. Technologically, the SGC focuses on the interaction of proteins with small molecules (ligands, inhibitors, substrates and co-factors), and on the coverage of protein families.
Background vectors
Click here for further information on the vectors used in the SGC clone collection and details on how to purchase these vectors.
The SGC expression clone collection contains clones from a variety of families. The common denominator is that they were all expressed and purified from bacteria and were used for structure determination. The proteins in general do not correspond to the full-length genes (transcripts) and in many cases proteins from the SGC collection represent domains of interest such as catalytic domains and protein-protein interaction or ligand binding domains with a focus on non-membrane protein domains.
Oxford_SGC_Clones_web
Stockholm_SGC_Clones_web
The Structural Genomics Consortium operates in the Universities of Oxford and Toronto and at the Karolinska Institute in Stockholm. The different SGC nodes share the core technologies but investigate non-overlapping target areas. The focus of the Oxford node includes protein kinases, phosphatases (protein tyrosine phosphatases) , oxidoreductases and other metabolic enzymes, as well as small G-proteins and signal transduction proteins (RGS, SOCS, 14-3-3, PDZ domains) together with lysine demethylases and DNA helicases.
The SGC clone collection comprises clones that allow expression of milligram quantities of purified protein using the host strains and protocols indicated in the respective structure pages of the SGC website.
These proteins provide a rich resource for biochemical investigations, including crystallisation and biophysical characterisation, small-molecule inhibitor screens, ligand/drug screening and generation of antibodies.
The SGC expression clone collection is not currently searchable via our GenomeCUBE® search and cannot be ordered online. We are in the process of integrating the data, however in the meantime you can still order these clones using a purchase order sent by email or fax to our customer services at the contact details below.
To place your order:
1. Search for the clone you require in the databases at the links below and identify the Construct ID
Oxford_SGC_Clones_web
Stockholm_SGC_Clones_web
If you have any problems finding the clone you are looking for please contact us.
2. Use the Construct ID to place your purchase order
Price per single clone £87
Price for entire library £950
(Prices exclude VAT and shipping)
For pricing on multiple clones please contact our sales team for a quotation.
3. Print off, read and sign the appropriate Material Transfer Agreement
US-only
non-US
4. Send your order and signed Material Transfer Agreement to
Fax: +44 (0) 115 973 9021
Background vectors
Click here for further information on the vectors used in the SGC clone collection and details on how to purchase these vectors.
Expression clone generation
Expression clones were generated by PCR and ligation-independent cloning (LIC) into one or more of a set of vectors. The first choice of vector is pNIC28-Bsa4, with the expression of the cloned gene driven by the T7-LacO system. Proteins cloned in this vector are fused to an amino-terminal tag of 23 residues (MHHHHHHSSGVDLGTENLYFQ*SM) including a hexahistidine (His6) and a TEV-protease cleavage site. Several alternative expression vectors have been used with selected targets. The standard hosts for bacterial expression are derivatives of BL21(DE3) providing tRNAs for rare codons in the host cells. Specific host strains were created to co-express either accessory proteins for expression pf particular target proteins. Each clone in the collection has been verified by the SGC to express the expected protein from the appropriate bacterial host. The library and individual clones are supplied in the MACH-1 strain of E.coli along with BL21(DE3) expression strains (BL21(DE3)-R3 and BL21(DE3)-R3-pRARE2).
Please find all the technical information included in the annotated clone lists.
Protocols / Clone Handling
The full materials and methods (e.g. protein purification protocols) and additional construct information on a particular protein can be found on the SGC website
http://www.thesgc.org/science/constructs
References
Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown NA, Gileadi O. High-throughput production of human proteins for crystallization: the SGC experience. J Struct Biol. 172(1):3-13. (2010)
Gileadi O, Knapp S, Lee WH, Marsden BD, Muller S, Niesen FH, Kavanagh KL, Ball LJ, von Delft F, Doyle DA, Oppermann UC, Sundstrom M. The scientific impact of the Structural Genomics Consortium: a protein family and ligand-centered approach to medically-relevant human proteins J Struct Funct Genomics 15(10):1215-26 (2007)
For further information and prices please contact us or call +44 (0)115 973 9012