C. elegans ORF-RNAi Resource (Vidal)

The ORFeome-RNAi v1.1 library contains 11,511 RNAi clones, which targets 10,953 genes. 485 of these genes are targeted by 2 or more constructs. The library covers the potential knockdown of 55% of the 19,920 unique protein-coding genes predicted in "worm sequence" 112.

This includes ~1,700 unique genes not previously targeted by existing RNAi feeding libraries (an additional ~9% of the worm genome). In conjunction with our Arhinger RNAi feeding library, the overall predicted coverage of worm genes is ~86%.

As part of our sequence verification service, individual clones are end sequenced before they are sent to you. If the requested clone does not match the expected sequence, Source BioScience will not apply any charge and will contact the purchaser to confirm if they wish to cancel the order.This risk free option ensures that you have the right clone, removing the chance of receiving one of the wrong clones that are inherent in some libraries and can also save you time from having to sequence the clone yourself.

PLEASE NOTE – That Source Bioscience excludes any warranty pertaining to the accuracy of the sequence for clones orders where the client chooses to reject the offered sequence verification option, as the clones are supplied as-is, direct from the original library.

  • Individual clones
  • Subsets of your chosen clones from the collection

Protocols / Clone Handling

Please note that the clone(s) are tested as negative in our phage contamination assay prior to shipping. However, these clones should still be handled with care as no phage assay can be guaranteed to be 100% accurate.

Plates contain LB broth with 8% glycerol and ampicillin. The plates are sent on Dry Ice, and should be stored at -70 °C.

Individual clone pools have been streaked onto LB agar containing ampicillin (100 µg/ml). Please store them at 4 °C (not in a freezer). As soon as possible, transfer the culture to LB broth containing ampicillin + 8% glycerol and incubate overnight at 37 °C (do not try to transfer single colonies, instead scoop out the whole bacterial culture using a disposable inoculation loop). Incubate overnight at 37 °C for subsequent freezing at -70 °C.

The HT115 (DE3) genotype is as follows:
F-, mcrA, mcrB, IN(rrnD-rrnE)1, lambda -, rnc14::Tn10(DE3 lysogen: lavUV5 promoter -T7 polymerase) (IPTG-inducible T7 polymerase) (RNase III minus).

The universal primers that can be used for PCR amplification and sequencing are:

These clones are for research purposes only.

For further information and prices please contact us or call +44 (0)115 973 9012