C. elegans ORF-RNAi Resource (Vidal)
The ORFeome-RNAi v1.1 library contains 11,511 RNAi clones, which targets 10,953 genes. 485 of these genes are targeted by 2 or more constructs. The library covers the potential knockdown of 55% of the 19,920 unique protein-coding genes predicted in "worm sequence" 112.
This includes ~1,700 unique genes not previously targeted by existing RNAi feeding libraries (an additional ~9% of the worm genome). In conjunction with our Arhinger RNAi feeding library, the overall predicted coverage of worm genes is ~86%.
As part of our sequence verification service, individual clones are end sequenced before they are sent to you. If the requested clone does not match the expected sequence, Source BioScience will not apply any charge and will contact the purchaser to confirm if they wish to cancel the order.This risk free option ensures that you have the right clone, removing the chance of receiving one of the wrong clones that are inherent in some libraries and can also save you time from having to sequence the clone yourself.
PLEASE NOTE – That Source Bioscience excludes any warranty pertaining to the accuracy of the sequence for clones orders where the client chooses to reject the offered sequence verification option, as the clones are supplied as-is, direct from the original library.
- Individual clones
- Subsets of your chosen clones from the collection
The library has many applications including but not limited to:
- High throughput screening
- Individual knock down experiments
Order Conditions
The use of the resource is limited to research purposes. The collection is subject to the following MTA and our clone terms and conditions.
Due to MTA requirements, individual clones and libraries should be ordered via our website.
To order individual clones, download the library database, and use our product code (e.g. DFCIp3320A0711054D) to search and order at the link below:
The entire library can be ordered below:
| Product Code |
Name |
Academic |
Commercial |
|
| 3320_Cel_ORF_RNAi |
C.elegans ORF-RNAi |
£8,200.00
|
£8,200.00
|
|
| Cross Reference |
Species |
Caenorhabditis elegans |
| Technical Clone Data |
Library |
C .elegans ORF-RNAi (Vidal) |
| Source |
Species: Caenorhabditis elegans |
| Vector |
pL4440_DEST |
| Host |
Host Species: E. coli; Host Strain: HT115(DE3) |
| Growth Conditions |
Growth Conditions medium: LB; Growth Conditions antibiotic: Amp (100 µg/ml) |
Protocols / Clone Handling
Please note that the clone(s) are tested as negative in our phage contamination assay prior to shipping. However, these clones should still be handled with care as no phage assay can be guaranteed to be 100% accurate.
Plates contain LB broth with 8% glycerol and ampicillin. The plates are sent on Dry Ice, and should be stored at -70 °C.
Individual clone pools have been streaked onto LB agar containing ampicillin (100 µg/ml). Please store them at 4 °C (not in a freezer). As soon as possible, transfer the culture to LB broth containing ampicillin + 8% glycerol and incubate overnight at 37 °C (do not try to transfer single colonies, instead scoop out the whole bacterial culture using a disposable inoculation loop). Incubate overnight at 37 °C for subsequent freezing at -70 °C.
The HT115 (DE3) genotype is as follows:
F-, mcrA, mcrB, IN(rrnD-rrnE)1, lambda -, rnc14::Tn10(DE3 lysogen: lavUV5 promoter -T7 polymerase) (IPTG-inducible T7 polymerase) (RNase III minus).
The universal primers that can be used for PCR amplification and sequencing are:
pL4440-dest-RNAi-FOR (5'-GTTTTCCCAGTCACGACGTT-3')
pL4440-dest-RNAi-REV (5'-TGGATAACCGTATTACCGCC-3')
These clones are for research purposes only.
References
References & Acknowledgments
Members of Marc Vidal's lab (Dana-Farber Cancer Institute) & Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
Rual JF, Ceron J, Koreth J, Hao T, Nicot AS, Hirozane-Kishikawa T, Vandenhaute J, Orkin SH, Hill DE, van den Heuvel S, Vidal M. "Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library". Genome Res. 2004 Oct;14(10B):2162-8. Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
For further information and prices please contact us or call +44 (0)115 973 9012