GeneCopoeia OmicsLink™ shRNA Clone Collections

GeneCopoeia OmicsLink™ shRNA clone collections include lentiviral and non-viral vector-based shRNA constructs against genome-wide human, mouse and rat genes. shRNA of varying lengths (19 to 29 bases) were designed using a proprietary algorithm to make shRNA expression constructs that have high knockdown efficiency with minimal off-target effect

  • Guaranteed shRNA knockdown. A set of four expression constructs is offered against every target gene with the guarantee that at least one of the four will have a knockdown effect of 70% or more on corresponding gene expression as determined by qRT-PCR, otherwise the constructs will be replaced one time free of charge. For more about our guaranteed shRNA knockdown, refer to warranty and cancellation policy here.
  • All cell types covered, Lentiviral and non-viral vector options allow transfection or transduction of shRNA into difficult-to-transfect cells as well as more conventional cell lines.
  • Multiple delivery formats shRNAs are delivered as 4 individual constructs of 5 µg purified plasmid. Lentiviral particles for shRNA are also available as a custom service (contact us for further information).
  • Markers and reporters Vectors with mCherry or eGFP reporter genes for monitoring transfection or transduction efficiencies. Stable cell selection with puromycin marker.
  • Fully sequenced expression cassettes. Expression cassettes of all shRNA clones are fully sequenced including the promoter, sense and antisense target sequences, hairpin, terminator, and other linker sequences.

 

Lentiviral and non-viral expression vector-based shRNA clones

Figure 1. Lentiviral and non-viral expression vector-based shRNA clones

A set of four expression constructs is offered against every target gene with the guarantee that at least one of the four will have a knockdown effect of 70% or more on corresponding gene expression as determined by qRT-PCR, otherwise the constructs will be replaced one time free of charge. For more about our guaranteed shRNA knockdown, refer to warranty and cancellation policy here.

More information can be found here OmicsLink™ shRNA Clone Collections.

Protocols / Clone Handling

Protocol information can be viewed here.

References

GeneCopoeia shRNA Clone Collections publications.


For further information and prices please contact us or call +44 (0)115 973 9012