Low Ionic Strength Saline Additive Reagent (LISS2)
Some laboratories find it convenient to make a simple addition of a LISS reagent to the washed cells and serum. The addition of 2 drops of LISS 2 to a mixture of red cells and serum provides a low ionic environment, which may enhance antigen/antibody reactions. When using LISS 2, there is no requirement to wash the red cells and support them in standard LISS solution.
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