Basket

You have nothing in your cart

Human Domain Antibody Library (DAb)

Human Domain Antibody Library (DAb)

We are pleased distribute the Domain Antibody Phagemid library constructed by Dr Daniel Christ (formally of the MRC Laboratory of Molecular Biology, Cambridge UK and currently based at the Garvan Institute, Sydney, Australia).

This library is based on a VH framework (V3-23/D47). Diversity was introduced into the antigen binding domains by PCR mutagenesis into CDR1, CDR2 and CDR3. There are many benefits to this library.

  • Produced binders to the model antigens refold without major loss of antigen-binding activity upon cooling.
  • Antibodies selected maintain antigen-binding activity after up to 25 cycles of heat denaturation, rivalling the heat resistance of thermophilic protein such as Taq polymerase.
  • Repertoire withstands heat-induced aggregation on phage.
  • These benefits offer a wide range of diagnostic and therapeutic research applications and can further maximise the number of binders to your antigen of interest.

This library is supplied as a single kit containing:

  • Phagemid antibody library (~3x109)
  • Helper phage KM13
  • Host strain TG1Tr
  • Optimised antibody expression strain HB2151
  • Controls: anti-beta-galactosidase & anti-bovine ubiquitin antibodies
  • detailed protocol

References

Niccheri, F., Real-Fernàndez, F., Ramazzotti, M., Lolli, F., Rossi, G., Rovero, P. and Degl'Innocenti, D. (2014),Human recombinant domain antibodies against multiple sclerosis antigenic peptide CSF114(Glc).J. Mol. Recognit., 27: 618–626. doi:10.1002/jmr.2386>

Lee CM, Iorno N, Sierro F, Christ D.
Selection of human antibody fragments by phage display.
Nat Protoc. 2007;2(11):3001-8.

Dudgeon K, Famm K, Christ D.
Sequence determinants of protein aggregation in human VH domains.
Protein Eng Des Sel. 2008 Oct 28.

Famm K, Hansen L, Christ D, Winter G.
Thermodynamically stable aggregation-resistant antibody domains through directed evolution.
J Mol Biol. 2008 Feb 29;376(4):926-31.

Christ D, Famm K, Winter G.
Repertoires of aggregation-resistant human antibody domains.
Protein Eng Des Sel. 2007 Aug;20(8):413-6.


Christ D, Famm K, Winter G.
Tapping diversity lost in transformations--in vitro amplification of ligation reactions.
Nucleic Acids Res. 2006;34(16):e108.


For further information and prices please contact us or call +44 (0)115 973 9012

©2017 Source BioScience. All rights reserved