Basket

You have nothing in your cart

Xenopus tropicalis cDNA Collections

Xenopus tropicalis cDNA Collections

The Xenopus tropicalis EST Project is a joint collaboration between the Sanger Institute and the Wellcome Trust/Cancer Research UK Gurdon Institute with the aim of supporting the X. tropicalis genetics and genomics effort. Source BioScience LifeSciences are supplying clones from four Xenopus (Silurana) tropicalis cDNA libraries:

X. tropicalis neurula cDNA library - TNeu
X. tropicalis gastrula cDNA library - TGas
X. tropicalis egg cDNA library - TEgg
X. tropicalis tadpole cDNA library - TTpa
X. tropicalis tailbud Head cDNA library - THda
X. tropicalis tailbud cDNA library - TTba
X. tropicalis Full length cDNA set - TFL

Clones are supplied individually or as a complete set of full-length clones (Gilchrist et al., 2004*) (http://genomics.crick.ac.uk/online/xt-fl-db.html).

Source BioScience LifeSciences are pleased to announce that Mike Gilchrist of the Gurdon Institute, University of Cambridge, has released four new full-length Xenopus tropicalis plates for us to distribute. The 384-well plates are part of the Xt3 project and have been designated plates 21-24, within the overall collection. To search by gene name or blast click here.

If you require further information on the additional plates, or would like to become part of a "user" group, please contact https://www.crick.ac.uk/mike-gilchrist

For specific information concerning plate or clone ordering please contact us.

The Sanger Institute has Generated approximately 55,000 5' EST sequences from each X. tropicalis cDNA library.

The complete set of 9180 full length clones is contained in 24 384-well plates (Arraying details). Publications stemming from clones obtained from these libraries should reference Gilchrist et al.,2004.

Protocols / Clone Handling

The clones have been streaked onto LB agar containing 50g/ml ampicillin. Please store them at 4oC (not in a freezer).
As soon as possible, re-streak the clones onto the same medium and incubate overnight at 37oC to obtain single colonies.

A selection of at least 10 single colonies should be picked and grown in LB broth containing ampicillin + 8% glycerol for subsequent freezing at –70oC. These frozen stocks can then be re-grown for plasmid isolation and purification.

Streaking out for single colonies is essential in case of contamination or cross-contamination, or if modifications of the original clone have taken place as the library has been replicated. If there is a problem, the more single colonies you streak out, the more likely it is that you can retrieve the original construct. You can also isolate the original clone from a contaminated sample by following this procedure.

References

Gilchrist, M., Zorn, A. M., Voigt, J, Smith, J.C., Papalopulu, N. and Amaya, E. (2004). Defining a large set of full length clones from a Xenopus tropicalis EST project. Dev. Biol. 271: 498-516.


For further information and prices please contact us or call +44 (0)115 973 9012

©2017 Source BioScience. All rights reserved