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XGC (Xenopus Gene Collection)

XGC (Xenopus Gene Collection)

The Xenopus Gene Collection (XGC) project is trans-NIH initiative, which was launched, as a subproject of the existing Mammalian Gene Collection. The general approach is to acquire or construct high-quality cDNA libraries. The libraries are screened by sequencing the 5' end of the clones. Putative full open reading frames clones are identified, and finally, selected clones are full-length sequence verified. The goal of the project was to make available a complete set of these full-length cDNA clones in Xenopus laevisandXenopus tropicalis.

IRBG XGC X. laevis verified full-length cam cDNA 6 96 576 
IRBN XGC X. tropicalis full length amp cDNA 40 96 3,840
IRBH XGC X. laevis full length amp cDNA 96 96 9,216
IRBJ MGC Xenopus full length cDNA  7 384 1,999

Protocols / Clone Handling

Clones should be stored at +4°C on receipt. As soon as possible, re-streak the clones onto the same medium and incubate overnight at 37°C to obtain single colonies.

A selection of at least 10 single colonies should be picked and grown in LB broth containing the appropriate antibiotic and 8 % glycerol for subsequent freezing at -70°C.

These frozen stocks can then be re-grown for plasmid isolation and purification.

References

R. D. Morin et al., Sequencing and analysis of 10,967 full-length cDNA clones from Xenopus laevis and Xenopus tropicalis reveals post-tetraploidization transcriptome remodeling. Genome Res. 16(6), 796-803 (June 2006).
MGC Project Team, The status, quality, and expansion of the NIH Full-length cDNA Project: The Mammalian Gene Collection (MGC). Genome Res. 14(10B), 2121-2127 (October 2004).
R. L. Strausberg et al., The Mammalian Gene Collection. Science. 286(5439), 455-457 (15 October 1999).


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