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Arabidopsis Thaliana Transcription Factor ORF (REGIA)

Arabidopsis Thaliana Transcription Factor ORF (REGIA)

Transcription factors (TFs) are regulatory proteins that have played a pivotal role in the evolution of eukaryotes and that also have great biotechnological potential. REGIA (REgulatory Gene Initiative in Arabidopsis) is an EU-funded project involving 29 European laboratories with the objective of determining the function of virtually all transcription factors from the model plant, Arabidopsis thaliana. 

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447270/pdf/CFG-03-102.pdf

Approximately 1000 clones:

https://www.ncbi.nlm.nih.gov/nucest/?term=MPIZ-ADIS-065d
http://www.gabipd.org/materials/fullORFArabidopsis.shtml

Protocols / Clone Handling

Plates contain LB broth with 8% glycerol and antibiotic* and are sent on dry ice. These should be stored at -70°C. Individual clones have been streaked onto LB agar containing antibiotic*. Please store them at 4°C (not in a freezer). As soon as possible, re-streak the clones onto the same medium and incubate overnight at 37°C to obtain single colonies.


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