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Promoterome Resources

Promoterome Resources

Following on from the success of the C. elegans ORFeome library, Marc Vidal and his lab, have given us access to distribute his C. elegans Promoterome library v1.1.

The development of systems biology requires the characterisation of expression profiles. Interpretation of such results should allow for the assessment of gene expression in cells and tissues, under different developmental stages and environmental conditions. The library includes clones containing ~6,000 predicted promoters, cloned into the highly flexible MultiSite Gateway™ system (Invitrogen). These promoters can be transferred easily into various Gateway ™ Destination vectors to drive expression of markers such as GFP alone (promoter::GFP constructs), or in fusion with protein-encoding open reading frames available in ORFeome resources (promoter::ORF::GFP).

Source BioScience offers, for research purposes only, individual clones from a single well, a subset of clones or the complete set of 71 plates (96-well format). 

We can also make subsets of your chosen clones from the collection. Please contact us to discuss your requirements.

 

Protocols / Clone Handling

Please note that the clone(s) are tested as negative in our phage contamination assay prior to shipping. However, these clones should still be handled with care as no phage assay can be guaranteed to be 100% accurate.

A selection of at least 10 single colonies should be picked and grown in LB broth containing the appropriate antibiotic + 8 % glycerol for subsequent freezing at -70°C.
These frozen stocks can then be regrown for plasmid isolation and purification.

Streaking out for single colonies is essential in case of contamination or cross-contamination, or if modifications of the original clone have taken place as the library has been replicated. If there is a problem, the more single colonies you streak out, the more likely it is that you can retrieve the original construct. You can also isolate the original clone from a contaminated sample by following this procedure.

References

Genome Res. 2004 Oct;14(10B):2169-75.
"A first version of the Caenorhabditis elegans Promoterome."
Dupuy D, Li QR, Deplancke B, Boxem M, Hao T, Lamesch P, Sequerra R, Bosak S, Doucette-Stamm L, Hope IA, Hill DE, Walhout AJ, Vidal M.
Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.

Acknowledgements and Subsequent Publication:

We are grateful to Dr. Marc Vidal (Dana-Farber Cancer Institute, Harvard Medical School, Boston) for the gift of this library and associated database.

Please acknowledge the Dana-Farber Cancer Institute and Source BioScience in any resulting publications, clones should be described in the paper in their ID form eg T22D1.3

Please also cite the following paper:

Dupuy D, Li QR, Deplancke B, Boxem M, Hao T, Lamesch P, Sequerra R, Bosak S,
Doucette-Stamm L, Hope IA, Hill DE, Walhout AJ, Vidal M.
“A first version of the Caenorhabditis elegans Promoterome.”
Genome Res. 2004 Oct;14(10B):2169-75.


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