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Chicken Genomic Clone Collection

Chicken Genomic Clone Collection

This chicken BAC library was constructed by Richard Crooijmans from Martien Groenen's group at the Wageningen Agricultural University, Department of Animal Sciences, Animal Breeding and Genetics Group, The Netherlands in collaboration with the Texas A&M BAC Centre of the Texas A&M University, College Station, Texas, USA . Work on this resource is co-ordinated by Richard Crooijmans (richard.crooijmans@alg.vf.wau.nl) and by ordering this library you are agreeing to your name being passed on to him.

Female White Leghorn Chicken blood genomic DNA was cloned into the pECBAC1 vector. This vector is a modification of pBeloBAC11  containing a unique EcoR1 site, the EcoR1 site within the vector having been removed leaving only one EcoR1 site within the lacZ gene (Frijters et al Theor. Appl. Genet. [1997] 94:390-399). The average insert size is 130 kb. The library consists of approx. 50,000 clones providing a 5.4-fold genomic coverage.

130 x 384 plates containing  49,920 clones.

Protocols / Clone Handling

Clones are streaked onto LB agar containing chloramphenicol (12.5µg/ml).

To use restreak the clone onto the same agar type, in such a way as to obtain single colonies. Incubate at 37°C overnight.
Single colonies are then ready for DNA isolation, if required, using the accompanying protocol which although described for PAC clones also works for BAC clones.

Clones can be grown in LB broth + chloramphenicol, and glycerol added after growth to a concentration of 25% for long term storage at -70°C.

References

Crooijmans RP1, Vrebalov J, Dijkhof RJ, van der Poel JJ, Groenen MA.
Two-dimensional screening of the Wageningen chicken BAC library. Mamm Genome. 2000 May;11(5):360-3.

Please acknowledge the originators of the library, Richard Crooijmans et al, and Source BioScience for providing the clone in any publication arising out of using this resource. An example of how a clone (E.g. 101-m19) should be described in a publication is: 101m19 from the Chicken BAC library.


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