The Fugu rubripes BAC library, cloned into pBeloBACII, was constructed at Incyte using Fugu genomic DNA supplied by Dr Greg Elgar (School of Biological Sciences, Queen Mary, University of London, Mile End Road, London, E1 4NS ).
This library - along with the cosmid library also available through Source BioScience LifeSciences - underpins and is being integrated into the Fugu genome draft assembly. The data will be linked together to form the long-range physical map of the Fugu genome.
All the data linking Fugu draft assembly to BAC and cosmid clones is available from the Fugu website, allowing selection of genomic clones from any given region of the Fugu genome for further investigation or sequence finishing.
The library contains 42,624 clones arrayed in 111 (384-well) plates. The library plates are numbered from 176-286.
Over half (23,453) of these clones have been HindIII fingerprinted and have an average insert size of 80kb, extrapolating to a nine-fold coverage of the genome.
The characteristics of the Fugu genome, along with the large evolutionary distance between bony fish and mammals make Fugu a useful tool for studying gene evolution
The use of the resource is limited to research purposes. The collection is subject to the following MTA and our clone terms and conditions.
Due to MTA requirements, individual clones and libraries should be ordered via our website.
For individual clone orders please use our website search engine:
Examples of clone nomenclature (Please note: no leading '0' in well location):
Fugu BAC: RC191-P16, RC191-P4 or BAC235-K10
How to find Fugu BAC clones
Find the genomic sequence of your region of interest from Ensembl and use the BLAST search at the link below, selecting the Fugu BAC ends from the database dropdown on the tool.
The search result shows lines connecting the BAC ends spanning the area of interest.
The BAC end sequences can be found by clicking on the links in the allignment results. To find them on our website you have to remove the x1 or y1 and the Fb:b, and remove any 0s from the plate location after the letter e.g. Fb:b186L04x1 becomes RC186-L4, Fb:b182C11 becomes RC182-C11. If you cannot find the clone using the RC prefix then try BAC as a prefix.
Please contact us if you still have difficulty in finding the clone.
Source Species: Takifugu rupides
Vector size: 7507bp
Antibiotic resistance: chloramphenicol (20 µg/ml).
Protocols / Clone Handling
Please note that the clone(s) are tested as negative in our phage contamination assay prior to shipping. However, these clones should still be handled with care as no phage assay can be guaranteed to be 100% accurate.
Clones are streaked onto LB agar containing chloramphenicol (20 µg/ml).
To use: re-streak the bacteria for single colonies on the same agar type, incubating overnight at 37°C.
Single colonies are then ready for DNA isolation, if required.
Clones can be grown in LB broth + chloramphenicol, and glycerol added after growth to a concentration of 25% for long term storage at -70°C.
Please acknowledge the originator of the library, Dr. Greg Elgar at the UK HGMP Resource Centre, for providing the clone in any publication arising out of using this resource. Clones (e.g. 202-P6) should be cited as: "B202p06 from the Fugu BAC library (Dr. G. Elgar, UK HGMP Resource Centre)".
For further information and prices please contact us or call +44 (0)115 973 9012