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Fugu Cosmid

Fugu Cosmid

The Fugu library provided by the Resource Centre was constructed by Elgar and Nizetic (unpublished data). It has an average insert size of of 40 Kb and consists of approximately 76,000 clones. The vector used is Lawrist 4. More information about the library may be obtained from the Genome News, 20 (1995).

G.Elgar and D.Nizetic, unpublished data.

The library contains 52,992 clones arrayed in 138 (384-well) plates. 

Protocols / Clone Handling

Clones are streaked onto LB agar containing kanamycin (25µg/ml).

To use: restreak the clone onto the same agar type, in such a way as to obtain single colonies. Incubate at 37°C overnight.
Single colonies are then ready for DNA isolation, if required, using the accompanying protocol.

Clones can be grown in LB broth + kanamycin, and glycerol added after growth to a concentration of 25% for long term storage at -70°C.

References

Please acknowledge the originator of the library, Elgar and Nizetic, and Source BioScience LifeSciences for providing the library in any publication arising out of using this resource. An example of how a clone (E.g. 171-m19) should be described in a publication is: 171m19 from the Elgar Fugu library.

Elgar G, Clark MS, Meek S, Smith S, Warner S, Edwards YJ, Bouchireb N, Cottage A, Yeo GS, Umrania Y, Williams G, Brenner S. Generation and analysis of 25 Mb of genomic DNA from the pufferfish Fugu rubripes by sequence scanning.Genome Res. 1999 Oct;9(10):960-71.


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