Human BAC (RPCI-11)
The RP11 Human Male BAC library was generated by the BACPAC Resource Center (BPRC) at the Children’s Hospital Oakland Research Institute by Kazutoyo Osoegawa. The work was funded by the National Human Genome Research Institute (NHGRI, NIH).
Male blood DNA was partially digested using a combination of EcoRI and EcoRI Methylase. The size selected DNA was then cloned into the pBACe3.6 vector). For Segment 5 the same DNA was partially digested with MboI. This was then size selected and ligated into the cloning vector pTARBAC1. The products were then transformed into E.coli DH10B. The clones have been arrayed into 384 well plates.
Source BioScience offers the complete clone set of 1440 384-well microtitre plates or individual clones, for research purposes only. We can also make subsets of your chosen clones from the collection. Please contact us to discuss your requirements.
- FISH - Fluorescent In Situ Hybridization - analysis of chromosomal rearrangements
- Matrix-CGH Comparative Genomic Hybridization - high resolution analysis of genomic DNA
- Protein - DNA binding studies - promotor, enhancer, silencer studies
- Subcloning of control elements
Due to MTA requirements, RPCI-11 clones and libraries should be ordered online via our website, using the clone ID format e.g. RP11-96I16 to search for individual clones. Search using the box below:
The use of the resource is limited to research purposes. The collection is subject to the following MTA and our clone terms and conditions.
We recommend using the public databases to find the RPCI-11 clone you are looking for, click here for advice on how to search for your clone.
The entire RPCI-11 library can be ordered below:
||Human BAC (RPCI-11)
Library Human BAC (RPCI-11)
Source Species: Homo sapiens; Sex: male; Tissue: blood
Cloning Site 3s: EcoRI; Cloning Site 5s: EcoRI
Cloning Site 3s: BamHI; Cloning Site 5s: BamHI
Host Species: E. coli; Host Strain: DH10B
Growth Conditions medium: LB; Growth Conditions antibiotic: Cam (30 µg/ml)
Protocols / Clone Handling
Clones are streaked onto LB agar containing chloramphenicol (20µg/ml) and should be stored at 4°C on arrival.
Plates contain LB broth with 8% glycerol and the appropriate antibiotic. The plates are sent on Dry Ice, and should be stored at -70 °C.
Restreak the clone onto the same agar type, in such a way as to obtain single colonies. Incubate at 37°C overnight.
Single colonies are then ready for DNA isolation, if required, using this protocol which although described for PAC clones also works for BAC clones.
Clones can be grown in LB broth + chloramphenicol, and glycerol added after growth to a concentration of 25% for long term storage at -70°C.
For any publications arising from the use of these clones please acknowledge the originators of the library, Kazutoyo Osoegawa, Minako Tateno and Pieter deJong, and Source BioScience for providing the clones in any publication arising out of using this resource.
- Osoegawa, K., Mammoser, A. G., Wu, C., Frengen, E., Zeng, C., Catanese, J. J., de Jong, P. J. (2001) A Bacterial Artificial Chromosome Library for Sequencing the Complete Human Genome, Genome Research, Vol. 11, Issue 3, 483-496, March 2001
- Osoegawa, K., Woon, P.Y., Zhao, B., Frengen, E., Tateno, M., Catanese, J.J, and de Jong, P.J. (1998) An Improved Approach for Construction of Bacterial Artificial Chromosome Libraries, Genomics 52, 1-8; Article # GE985423.
For further information and prices please contact us or call +44 (0)115 973 9012