Human Minimal Genomic Set
The Minimal Genomic Clone Set was developed on basis of NCBI's genome assembly and the Ensembl annotation. The current clone set consists of 25,130 clones. The majority (22,898 clones, 91%) of which have been completely sequenced during the human genome sequencing project. Gaps were covered by 2,232 end-sequenced clones as far as such clones were available. We focused on publicly available BAC and PAC clones and completed the set where necessary with cosmids. The overall average insert size is approx 130 kb.
- Clone Collection in microtiter plates
- Customized subsets
Genomic Clones are a valuable resource for the functional analysis of genes, they can be used for a multitude of applications:
- FISH - Fluorescent In Situ Hybridization - analysis of chromosomal rearrangements
- Matrix-CGH Comparative Genomic Hybridization - high resolution analysis of genomic DNA
- Protein - DNA binding studies - promotor, enhancer, silencer studies
- Subcloning of control elements
Protocols / Clone Handling
Individual clones have been streaked onto LB agar containing the appropriate antibiotic. Please store them at 4 °C (not in a freezer).
As soon as possible, transfer the culture to LB broth containing the antibiotic + 8% glycerol and incubate overnight at 37 °C (do not try to transfer single colonies, instead scoop out the whole bacterial culture using a disposable inoculation loop).
Incubate overnight at 37 °C for subsequent freezing at -70 °C.
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