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Human TilePath Genomic Set

Human TilePath Genomic Set

The fully indexed Human Tilepath library, constructed at Roswell Park Cancer Institute and The Wellcome Trust Sanger Institute is a genomic clone collection of FISH mapped clones are displayed on the Ensembl genome browser.

The library was constructed as part of the Human genome project. To divide the complexity of the Human genome into reduced, workable elements, large DNA segments are cloned into either Bacterial or P1 derived artificial chromosomes. 

 

Source BioScience offers the complete clone set of 96 384-well microtitre plates or individual clones, for research purposes only. Alternatively, the plates have been re-arrayed such that each of the chromosomes can be ordered individually.

Protocols / Clone Handling

Individual clones have been streaked onto LB agar containing the appropriate antibiotic. Please store them at 4 °C (not in a freezer).

As soon as possible, transfer the culture to LB broth containing the antibiotic + 8% glycerol and incubate overnight at 37 °C (do not try to transfer single colonies, instead scoop out the whole bacterial culture using a disposable inoculation loop).

Incubate overnight at 37 °C for subsequent freezing at -70 °C.

Plates contain LB broth with 8% glycerol and the appropriate antibiotic. The plates are sent on Dry Ice, and should be stored at -70 °C.

References

Acknowledgements and subsequent publication:

Osoegawa K, Mammoser AG, Wu C, Frengen E, Zeng C, Catanese JJ, de Jong PJ. A bacterial artificial chromosome library for sequencing the complete human genome. Genome Res. 2001 Mar;11(3):483-96.

The Wellcome Trust Sanger Institute, Hixton Campus, Cambridge and Roswell Park Cancer Institute, Buffalo, New York.


For further information and prices please contact us or call +44 (0)115 973 9012

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