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Daniokey Pilot BAC clones

Daniokey Pilot BAC clones

The Daniokey Pilot BAC library was created by the Ronald Plasterk Laboratory and Keygene N.V. The library provides approximately 1x coverage of the Zebrafish genome.

The library was created from the Tübingen zebrafish strain male testis DNA, provided by R. Geisler. The DNA was partially digested with HindIII. The DNA was cloned into the pIndigoBAC-536 vector, in E.coli DH10B.

There are 11,808 clones with an average insert size of around 130kb.

Source BioScience offers the complete clone set of 31 384-well microtitre plates or individual clones, for research purposes only. We can also make subsets of your chosen clones from the collection. Please contact us to discuss your requirements.

Protocols / Clone Handling

Plates: contain LB broth with 7.5% glycerol and chloramphenicol. These are sent on Dry Ice. These should be stored at -70°C.

Clones: are streaked onto LB agar containing chloramphenicol (12.5µg/ml).

To use: re-streak the clone onto the same agar type, in such a way as to obtain single colonies. Incubate at 37°C overnight. Clones can be grown in LB broth + chloramphenicol, and glycerol added after growth to a concentration of 25% for long-term storage at -70°C.

References

Suster, M.L., Abe, G., Schouw, A. and Kawakami, K. (2011). Transposon-mediated BAC transgenesis in zebrafish. Nat Protoc 6, 1998-2021
Howe, K, Clark, M.D. et al (2013) The zebrafish reference genome sequence and its relationship to the human genome Nature 496 (7446), 498-503

Subsequent publication:

Please acknowledge the originators of the library, R.Plasterk and Keygene N.V., and Source BioScience for providing the clones in any publication arising out of using this resource.


For further information and prices please contact us or call +44 (0)115 973 9012

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