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Zebrafish BAC (CHORI-211)

Zebrafish BAC (CHORI-211)

The CHORI-211 Zebrafish BAC library was generated by the BACPAC Resource Center (BPRC) at the Children’s Hospital Oakland Research Institute by Chung Li Shu and Kazutoyo Osoegawa in Pieter De Jong’s Laboratory. The library provides approximately 10x coverage of the Zebrafish genome.

The library was created from the Tübingen zebrafish strain male testis DNA, provided by Gerd-Joerg Rauch. The DNA was partially digested using a combination of EcoRI and EcoRI Methylase. The DNA was size selected and cloned into the pTARBAC2.1 vector, in E.coli DH10B.
There are 105,907 clones with an average insert size of around 165kb.

Source BioScience offers the complete clone set of 288 384-well microtitre plates or individual clones, for research purposes only. We can also make subsets of your chosen clones from the collection. Please contact us to discuss your requirements.

Protocols / Clone Handling

Plates: contain LB broth with chloramphenicol (20µg/ml) + 7.5% Glycerol. They are shipped on Dry Ice and should be stored at -70°C.

Clones: are streaked onto LB agar containing chloramphenicol (20µg/ml).

To use: re-streak the clone onto the same agar type, in such a way as to obtain single colonies. Incubate at 37°C overnight. Clones can be grown in LB broth + chloramphenicol, and glycerol added after growth to a concentration of 25% for long-term storage at -70°C.

These clones are for research only.

References

Rauch, G.-J., Granato, M. and Haffter, P. (1997). A polymorphic zebrafish line for genetic mapping using SSLPs on high-percentage agarose gels. Technical Tips Online T01208.

Subsequent publication:

Please acknowledge the originators of the library, Robert Geisler and Pieter deJong, and Source BioScience for providing the clones in any publication arising out of using this resource.


For further information and prices please contact us or call +44 (0)115 973 9012

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