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Full ORF Expression Clones (ORFeome Collaboration - OC)

Full ORF Expression Clones (ORFeome Collaboration - OC)

Full ORF Expression Clones shuttled to order by Source BioScience.

To complement our extensive range of clones, we also offer ORFeome Collaboration clones in expression vectors, ready to express in  E. coli, yeast, the baculo system or mammalian cells.

We shuttle all ORFs into Gateway® expression (destination) vectors on demand and provide ready-to-express Full ORF Expression Clones. Various fusion tags such as His6, GST or V5 are available. All inserts are fully sequence-verified. We guarantee for the absence of frame shifts or stopcodons in frame.

The Gateway® Technology of Invitrogen Inc. is a universal cloning system that takes advantage of the site-specific recombination properties of bacteriophage lambda (Landy, 1989) to provide a rapid and highly efficient way to move your gene of interest into multiple vector systems.

We are continuously expanding our Full ORF Clone Collection. If a clone for your gene of interest is not available, please contact us

We offer any of the ORFeome Collaboration ORF entry clones in a ready to use expression format, including clones from:

  • OCAA and OCAB Human Full ORF Entry Clones - ORFeome Collaboration
  • OCAC Mouse Full ORF Entry Clones - ORFeome Collaboration
  • OCAF Zebrafish Full ORF Entry Clones - ORFeome Collaboration
  • XenORFeome  Xenopus Full ORF Entry Clones - ORFeome Collaboration
  • Drosophila ORFeome

Introduce this clone into the appropriate host (e.g. bacteria, yeast, baculovirus, mammalian cell) and express your protein of interest.
Many ORFs are available with or without stopcodon, and the availability of both formats provides the freedom of choice to produce native proteins or C-terminal fusion constructs.

Protocols / Clone Handling

Full ORF Expression Clones are supplied as bacterial culture and as purified DNA.

Please store the tubes with the bacterial culture at 4°C. The bacterial strain is DH10B. The bacterial strain provided cannot be used for protein expression.

Please check the respective functional elements of your expression construct match your preferred expression host (e.g. BL21 series where appropriate).
For eukaryotic expression constructs, please select a compatible yeast or mammalian cell line.

We cannot guarantee protein expression in your system of choice, but according to our experience the vector constructs are of very high quality. The protein coding sequence is at the optimal distance to the respective promoter element and in frame with the potential fusion tag.

References

More information on the creation of these libraries can be found in the following publications:

The ORFeome Collaboration Nature Methods 13:191–192 (2016) doi:10.1038/nmeth.3776.

Lamesch, P., Li, N, Milstein, S et al. (2006) hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. Genomics. 2007 Mar;89(3):307-15. Epub 2007 Jan 5. PMID: 17207965

Rual JF, Hirozane-Kishikawa T, Hao T, Bertin N, Li S, Dricot A, Li N, Rosenberg J, Lamesch P, Vidalain PO, Clingingsmith TR, Hartley JL, Esposito D, Cheo D, Moore T, Simmons B, Sequerra R, Bosak S, Doucette-Stamm L, Le Peuch C, Vandenhaute J, Cusick ME, Albala JS, Hill DE, Vidal M. Human ORFeome version 1.1: a platform for reverse proteomics. Genome Res 2004;14:2128-2135. PMCID: PMC528929


For further information and prices please contact us or call +44 (0)115 973 9012

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