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Human Expression Clones (SGC)

Human Expression Clones (SGC)

The Structural Genomics Consortium (SGC) provides a unique resource of verified Expression Clones which are suitable for generating well characterised and functionally active proteins or protein domains for various purposes.

Please note the SGC clones cannot currently be ordered via our website, please see below for details on how to order.

The Structural Genomics Consortium is a not-for-profit organisation set up to promote biomedical research by determining the structures of human proteins with potential therapeutic importance. Furthermore, the SGC investigates proteins from human parasites (mainly apicomplexan proteins). Thus the targets are selected based on their potential as drug targets or involvement in disease processes. The availability of high resolution structures constitutes the foundation for structure-based drug design projects. Technologically, the SGC focuses on the interaction of proteins with small molecules (ligands, inhibitors, substrates and co-factors), and on the coverage of protein families.

Background vectors

Click here for further information on the vectors used in the SGC clone collection and details on how to purchase these vectors.

The SGC expression clone collection contains clones from a variety of families. The common denominator is that they were all expressed and purified from bacteria and were used for structure determination. The proteins in general do not correspond to the full-length genes (transcripts) and in many cases proteins from the SGC collection represent domains of interest such as catalytic domains and protein-protein interaction or ligand binding domains with a focus on non-membrane protein domains.

Oxford_SGC_Clones_web
Stockholm_SGC_Clones_web

The Structural Genomics Consortium operates in the Universities of Oxford and Toronto and at the Karolinska Institute in Stockholm. The different SGC nodes share the core technologies but investigate non-overlapping target areas. The focus of the Oxford node includes protein kinases, phosphatases (protein tyrosine phosphatases) , oxidoreductases and other metabolic enzymes, as well as small G-proteins and signal transduction proteins (RGS, SOCS, 14-3-3, PDZ domains) together with lysine demethylases and DNA helicases.

Protocols / Clone Handling

The full materials and methods (e.g. protein purification protocols) and additional construct information on a particular protein can be found on the SGC website

http://www.thesgc.org/science/constructs

References

Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown NA, Gileadi O. High-throughput production of human proteins for crystallization: the SGC experience. J Struct Biol. 172(1):3-13. (2010)

Gileadi O, Knapp S, Lee WH, Marsden BD, Muller S, Niesen FH, Kavanagh KL, Ball LJ, von Delft F, Doyle DA, Oppermann UC, Sundstrom M. The scientific impact of the Structural Genomics Consortium: a protein family and ligand-centered approach to medically-relevant human proteins J Struct Funct Genomics 15(10):1215-26 (2007)


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