Basket

You have nothing in your cart

C. elegans TransgenOme Resource

C. elegans TransgenOme Resource

The C. elegans TransgeneOme Resource (MPI-CBG) is available exclusively from Source BioScience.

Developed by Dr Mihail Sarov in the TransgeneOmics Unit, Max Planck Institute of Molecular Cell Biology and Genetics, the C. elegans TransgenOme collection enables the in vivo expression of fluorescent- and affinity-tagged proteins in C. elegans, under endogenous cis regulatory control.

The TransgeneOme Project is dedicated to providing efficient platforms for high-throughput transgenesis in model organisms, and the group runs a portal designed as an open collaboration project for crowdsourcing strain generation, image acquisition and pattern annotation tasks.

  • Genome-wide resource of ~14700 tagged fosmid clones covering 73% of the protein-coding genes in C. elegans
  • Inserts comprise all important coding and regulatory sequences for most genes
  • C-terminal fusion protein expression construct - with a multipurpose tagging cassette consisting of GFP (fluorescence marker), Ty1 (flexible linker peptide) and 3xFLAG (affinity tag)
  • Genetic selection marker for worm transgenesis (unc-119) included
  • Constructs provided from the originator as non-clonal pools
  • Transgenic C. elegans lines will express fluorescent- and affinity- tagged proteins of interest under the native regulatory controls - enabling various tag-based functional assays
  • Advantages of the fosmid transgenes as gene expression reporters compared to customary promoter clones:
  • Inserts preserve the native cis regulatory elements including intronic and 3`UTR sequences
  • Expression of the tagged protein also reflects posttranscriptional regulatory mechanisms (e.g. rates of translation and protein degradation)
  • Web application available, providing full access to all construct-related information

Protocols / Clone Handling

Please note that the clone(s) are tested as negative in our phage contamination assay prior to shipping. However, these clones should still be handled with care as no phage assay can be guaranteed to be 100% accurate.

Download clone list for the whole C. elegans TransgeneOme Resource
Download list of C. elegans genes covered by the TransgeneOme Collection
Manuals from MPI webportal

References

Sarov. M., Murray. J., Schanze. K.., Pozniakovski. A., Niu. W., Angermann. K., Hasse. S., Rupprecht. M., Vinis. E., Tinney. M., Preston. E., Zinke. A., Enst. E., Teichgraber. T., Janette. J., Reis. J., Janosch. J., Schloissnig. S., Ejsmont. R.., Slightam. C., Xu. X., Kim. S., Reinke. V., Stewart. A., Snyder. M., Waterston. R., Hyman. A. (2012) A Genome-Scale Resource for In Vivo Tag-Based Protein Function Exploration in C. elegans. Cell. 150(4): 855-866.


For further information and prices please contact us or call +44 (0)115 973 9012

©2017 Source BioScience. All rights reserved