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Drosophila TransgenOme Resource

Drosophila TransgenOme Resource

The Drosophila TransgeneOme Resource (MPI-CBG) is available exclusively from Source BioScience.

Developed by Dr Mihail Sarov in the TransgeneOmics Unit, Max Planck Institute of Molecular Cell Biology and Genetics, the Drosophila TransgenOme Collection is the latest tool for the exploration of protein function in Drosophila melanogaster.

The tagged fosmid constructs can be easily used to generate transgenic fly lines expressing tagged proteins under native control, enabling various in vivo applications to investigate expression patterns, protein localisation and physical protein interactions.

  • Genome-scale collection of ~10000 validated clones covering ~9600 genes (73% of the Drosophila proteome)
  • Comprehensive genome-wide library of C-terminally tagged proteins within genomic fosmid constructs
  • Tagging cassette comprises affinity tags, protease sites and GFP suitable for protein localisation and complex purification studies
  • pFlyFos vector additionally comprises an inducible origin of replication (oriV) to allow a high copy number only for isolation of the fosmid DNA
  • Option to insert the fosmid constructs at a defined position into the fly genome as third copy reporter allele to generate transgenic animals*
  • Fosmid reporters can in principle recapitulate all aspects of gene expression regulation at transcriptional and post-transcriptional levels
  • Functional reagents that are able to substitute endogenous protein function
  • Web application available, providing full access to all construct-related information

Protocols / Clone Handling

Please note that the clone(s) are tested as negative in our phage contamination assay prior to shipping. However, these clones should still be handled with care as no phage assay can be guaranteed to be 100% accurate.

Download clone list for the whole Drosophila TransgeneOme Resource
Download list of Drosophila genes covered by the TransgeneOme Collection
Manuals from MPI webportal

References

Sarov M., Barz C., Jambor H., Hein M., Schmied C., Suchold D., Stender B., Janosch S., Vikas V., Krisnan R-T., Aishwarya K., Ferreira I.R.S., Ejsmont R.K., Finkl K., Hasse S., Kaempfer P., Plewka N., Vinis E., Schloissnig S., Knust E., Hartenstein V., Mann M., Ramaswami M., Raghavan K.V., Tomancak P., Schnorrer F. (2015) A genome-wide resource for the analysis of protein localisation in Drosophila preprint available on BioRxiv


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