Chicken Genomic Clone Collection
This chicken BAC library was constructed by Richard Crooijmans from Martien Groenen's group at the Wageningen Agricultural University, Department of Animal Sciences, Animal Breeding and Genetics Group, The Netherlands in collaboration with the Texas A&M BAC Centre of the Texas A&M University, College Station, Texas, USA . Work on this resource is co-ordinated by Richard Crooijmans ([email protected]) and by ordering this library you are agreeing to your name being passed on to him.
Female White Leghorn Chicken blood genomic DNA was cloned into the pECBAC1 vector. This vector is a modification of pBeloBAC11 containing a unique EcoR1 site, the EcoR1 site within the vector having been removed leaving only one EcoR1 site within the lacZ gene (Frijters et al Theor. Appl. Genet.  94:390-399). The average insert size is 130 kb. The library consists of approx. 50,000 clones providing a 5.4-fold genomic coverage.
130 x 384 plates containing 49,920 clones.
An invaluable tool for positional cloning and for comparative mapping studies.
The use of the resource is limited to research purposes. The collection is subject to an MTA and our clone terms and conditions.
Around 1/6th of the clones in this library have their end or full insert sequences published and mapped. This list of clones can be viewed here.
To find clones by gene name or genomic location use the NCBI Gallus gallus clone search and select WAG in the library selection list.
Unfortunately the nomenclature that we use in our clone search is different to that on NCBI so please find examples below of converted clone codes:
WAG-13O4 = WAU13-O4
WAG-13N9 = WAU13-N9
The vector used, pECBAC1, is a modification of pBeloBAC11, containing a unique EcoR1 site: the EcoR1 site within the vector having been removed leaving only one EcoR1 site within the lacZ gene (Frijters et al Theor. Appl. Genet.  94:390-399).
Protocols / Clone Handling
Clones are streaked onto LB agar containing chloramphenicol (12.5µg/ml).
To use restreak the clone onto the same agar type, in such a way as to obtain single colonies. Incubate at 37°C overnight.
Single colonies are then ready for DNA isolation, if required, using the accompanying protocol which although described for PAC clones also works for BAC clones.
Clones can be grown in LB broth + chloramphenicol, and glycerol added after growth to a concentration of 25% for long term storage at -70°C.
Crooijmans RP1, Vrebalov J, Dijkhof RJ, van der Poel JJ, Groenen MA.
Two-dimensional screening of the Wageningen chicken BAC library. Mamm Genome. 2000 May;11(5):360-3.
Please acknowledge the originators of the library, Richard Crooijmans et al, and Source BioScience for providing the clone in any publication arising out of using this resource. An example of how a clone (E.g. 101-m19) should be described in a publication is: 101m19 from the Chicken BAC library.
For further information and prices please contact us or call +44 (0)115 973 9012