Mouse BAC (bMQ)
We are pleased to distribute this fully indexed mouse BAC library, constructed in Alan Bradley′s lab, The Wellcome Trust Sanger Institute, Hinxton, UK.
The library was generated from AB2.2 ES cell DNA (129S7/SvEv Brd-Hprt b-m2) and represents 3.6 autosome and 1.24 sex chromosome coverage, with an average insert size of 110.68 kb. The clones cover over 97% of the mouse genome and 99.1 % of Ensembl genes. Finally, the BAC′s have been end-sequence profiled using SP6 and T7 primers, giving an average read-ends size of 739bp.
129s7/AB2.2 BAC clones are displayed on the Ensembl genome browser within a DAS (Distributed Annotation Server). The clones are displayed as green (the T7 reads from the telomere to the centromere) and pink (the T7 reads from centromere to telomere ), which indicates the orientation of the DNA insert in the vector, whilst end reads, are shown as grey bars.
We offer the complete clone set of 434 384-well microtitre plates or individual clones, for research purposes only. We can also make subsets of your chosen clones from the collection. Please contact us to discuss your requirements.
A library of this quality is ideal for the construction of gene targeting vectors. It may also be a useful tool for examining large-scale structural differences between 129v and other mouse strain genomes and facilitating high-throughput targeted manipulation of the mouse genome.
Due to MTA requirements, bMO clones and libraries should be ordered online using our website search below, using the clone ID format e.g. BMQ-38M6 to search for individual clones.
We recommend using the public databases to find the bMQ clone you are looking for, click here for advice on how to search for your clone.
The entire bMQ library can be ordered below:
||Mouse BAC (bMQ)
The use of the resource is limited to research purposes. The collection is subject to the following MTA and our clone terms and conditions.
Genomic DNA was partially digested with Sau3A1 and cloned into BamHI linearized pBACe3.6
Protocols / Clone Handling
Clones are streaked onto LB agar containing chloramphenicol (20μg/ml).
To use: re-streak the clone onto the same agar type, in such a way as to obtain single colonies. Incubate at 37°C overnight.
Clones can be grown in LB broth + chloramphenicol, and glycerol added after growth to a concentration of 8% for long-term storage at -70°C.
A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction. Adams DJ, Quail MA, Cox T, van der Weyden L, Gorick BD, Su Q, Chan WI, Davies R, Bonfield JK, Law F, Humphray S, Plumb B, Liu P, Rogers J, Bradley A. Genomics. 2005 Dec;86(6):753-8. Epub 2005 Oct 27. The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire CB10 1SA, UK.
For further information and prices please contact us or call +44 (0)115 973 9012