Mouse BAC (RPCI-23)
The RPCI-23 (RP23) BAC Library was constructed in Pieter de Jong's lab (Children's Hospital Oakland Research Institute) by visiting scientists Kazutoyo Osoegawa and Minako Tateno (RIKEN Tsukuba Life Science Center). From pooled tissues derived from three female C57BL/6J mice, kidney and brain genomic DNA samples were isolated and partially digested with a combination of EcoRI and EcoRI Methylase. Size-selected EcoRI fragments were cloned into the vector pBACe3.6 at the EcoRI sites. The ligation products were transformed into DH10B electro-competent cells (BRL Life Technologies).
- FISH - Fluorescent In Situ Hybridization - analysis of chromosomal rearrangements
- Matrix-CGH Comparative Genomic Hybridization - high resolution analysis of genomic DNA
- Protein - DNA binding studies - promotor, enhancer, silencer studies
- Subcloning of control elements
To order, please use the clone ID in our website search. Please note that due to MTA requirements we can only accept clone orders placed online.
We recommend using the public databases to find the RPCI-23 clone you are looking for, click here for advice on how to search for your clone.
The entire RPCI-23 library can be ordered below:
||Mouse BAC (RPCI-23)
The use of the resource is limited to research purposes. The collection is subject to the following MTA and our clone terms and conditions.
Library: RPCI-23 (RP23) DNA source: C57BL/6L kidney & brain Vector: pBACe3.6
Protocols / Clone Handling
Clones are streaked onto LB agar containing chloramphenicol (20µg/ml).
To use: restreak the clone onto the same agar type, in such a way as to obtain single colonies. Incubate at 37°C overnight.
Single colonies are then ready for DNA isolation, if required, using the accompanying protocol which although described for PAC clones also works for BAC clones.
Clones can be grown in LB broth + chloramphenicol, and glycerol added after growth to a concentration of 25% for long term storage at -70°C.
Osoegawa K, Tateno M, Woon PY, Frengen E, Mammoser AG, Catanese JJ, Hayashizaki Y, de Jong PJ.Bacterial artificial chromosome libraries for mouse sequencing and functional analysis. Genome Res. 2000 Jan;10(1):116-28.
For further information and prices please contact us or call +44 (0)115 973 9012