PULSin® enables you to study lethal proteins by controlling the level and time course of protein delivery into the cells. Similarly, delivery of blocking antibodies may provide additional information to traditional RNA interference experiments*. With PULSin® you can target intracellular proteins with antibodies in living cells without fixation.
* G. Cassinelli et al. (2006). Inhibition of c-Met and prevention of spontaneous metastatic spreading by the 2-indolinone RPI-1, Mol Cancer Ther 5:9, 2388-2397.
- Effective and robust delivery into the cytoplasm
- Delivery to a wide variety of cells including primary cells
- Highly efficient transfer
- Easy to use
Protein; Antibody; Peptide
Adherent & suspension cells grown in presence of serum
Number of transfection
0.4 ml of PULSin™ delivery reagent is sufficient to perform 25 delivery experiments in 6-well plates and 100 experiments in 24-well plates
Hepes Buffer (20mM) for protein dilution; R-Phycoerythrin used as a positive control.
PULSin: reagent for delivery of functional proteins & antibodies to living cells
For further information and prices please contact us or call +44 (0)115 973 9012